Human RNA is a diverse collection of ribonucleic acid molecules isolated from human biological specimens, including cells and tissues. It comprises messenger RNA (mRNA), ribosomal RNA (rRNA), transfer RNA (tRNA), and regulatory non-coding RNAs such as microRNAs (miRNAs). RNA abundance and composition provide information about gene-expression programs and cellular states and are therefore widely used to study gene expression, transcriptomes, and RNA-mediated regulation.
Key Features
-
Transcriptome representation: Total RNA or selected RNA fractions can support the analysis of coding and non-coding transcripts, depending on the isolation and enrichment strategy.
-
RNA quality and integrity: Purity, concentration, and RNA integrity are important parameters for reproducible downstream gene-expression analysis and RNA sequencing.
-
Human-derived material: Supports molecular studies based on RNA isolated from human cells, tissues, and other biological specimens.
Usual Research Applications
-
Reverse transcription quantitative PCR (RT-qPCR).
-
RNA sequencing (RNA-seq).
-
Transcriptome profiling.
-
Differential gene-expression analysis.
-
MicroRNA and non-coding RNA research.
-
Characterization of transcript abundance, transcript isoforms, and other RNA features using RNA-seq, depending on the experimental design.
-
Targeted quantitative measurement and validation of gene-expression changes using RT-qPCR.
