pEX-C-His Bacterial Expression Vector
Referência PS100076
Tamanho : 10ug
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pEX-C-His Bacterial Expression Vector
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PS100076
pEX-C-His, bacterial expression vector with C-terminal His tag, 10ug
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Citations
Small molecules inhibitors of the heterogeneous ribonuclear protein A18 (hnRNP A18): a regulator of protein translation and an immune checkpoint
Solano-Gonzalez, Coburn, Yu et al
Nucleic Acids Res (2021)
Solano-Gonzalez, Coburn, Yu et al
Nucleic Acids Res (2021)
Cited by 17
AI Summary: OriGene products contributed to this study by providing the hnRNPA1 Myc-DDK-tagged plasmid (RC203314) and the pEX-C-His vector, which were used to generate recombinant human His-hnRNP A1 for structural and functional analyses.
Snippet: recombinant human His-hnRNP A1 was obtained by subcloning the human open reading frame (NM_002136) from hnRNPA1 Myc-DDK-tagged plasmid (RC203314, OriGene Technologies Inc, Rockville, MD) into pEX-C-His vector (OriGene Technologies) in the BseR 1/Mlu1 restriction sites by PCR
Rhenium N-heterocyclic carbene complexes block growth of aggressive cancers by inhibiting FGFR- and SRC-mediated signalling
Domenichini, Casari, Simpson et al
J Exp Clin Cancer Res (2020) 39 (1), 276 DOI: 10.1186/s13046-020-01777-7
Domenichini, Casari, Simpson et al
J Exp Clin Cancer Res (2020) 39 (1), 276 DOI: 10.1186/s13046-020-01777-7
Cited by 27
AI Summary: OriGene’s pEX-Src-C-His plasmid was used to express recombinant Src protein, which was essential for studying the inhibitory effects of rhenium complexes on Src-mediated cancer signalling in this research.
Snippet: Recombinant Src was expressed in BL21-AI Escherichia coli (Thermo) containing the pEX-Src-C-His (Origene, Blue Heron Biotech) and purified in a modified procedure as previously described [30]
Annexin A7 is required for ESCRT III-mediated plasma membrane repair
Sønder, Boye, Tölle et al
Sci Rep (2019) 9 (1), 6726 DOI: 10.1038/s41598-019-43143-4
Sønder, Boye, Tölle et al
Sci Rep (2019) 9 (1), 6726 DOI: 10.1038/s41598-019-43143-4
AI Summary: OriGene's tGFP antibody (TA150041) was used for immunoprecipitation of tagged proteins, enabling the authors to study protein interactions involved in plasma membrane repair.
Snippet: … Proteins were immunoprecipitated from whole cell lysate using 3 µg antibody (tGFP, TA150041, Origene) coupled to protein A agarose beads (Millipore) or using RFP-Trap agarose beads (Chromotek) for View as HTML;... … Proteins were immunoprecipitated from whole cell lysate using 3 µg antibody (tGFP, TA150041, Origene) coupled to protein A agarose beads (Millipore) or using RFP-Trap agarose beads (Chromotek) for o/n at 4 °C according to the manufacturer’s instructions. …
Cloning, expression, and purification of the recombinant pro-apoptotic dominant-negative survivin T34A-C84A protein in Escherichia coli
Tsai, Chang, Sarvagalla et al
Protein Expr. Purif. (2019) 160, 73-83 DOI: 10.1016/j.pep.2019.04.003
Tsai, Chang, Sarvagalla et al
Protein Expr. Purif. (2019) 160, 73-83 DOI: 10.1016/j.pep.2019.04.003
AI Summary: OriGene’s pCMV6-AC-GFP/hSurvivin plasmid DNA (cat# RG205935) was used as the template for generating survivin mutants in this study, enabling the cloning and production of the recombinant pro-apoptotic survivin T34A-C84A protein.
Snippet: The pCMV6-AC-GFP/hSurvivin plasmid DNA (OriGene, cat# RG205935) was used as the SOE-PCR template for the generation of T34A-dNSur (contains an A-to-G substitution at nucleotide 100 that changes the threonine residue at amino acid 34 to an alanine) and;
Direct cysteine sulfenylation drives activation of the Src kinase
Heppner, Dustin, Liao et al
Nat Commun (2018) 9 (1), 4522 DOI: 10.1038/s41467-018-06790-1
Heppner, Dustin, Liao et al
Nat Commun (2018) 9 (1), 4522 DOI: 10.1038/s41467-018-06790-1
AI Summary: OriGene's pEX-Src-C-His plasmid was used to express recombinant Src and its variants in E. coli, enabling the biochemical studies that revealed how cysteine sulfenylation activates Src kinase.
Snippet: fer exciting new directions for drug discovery. Methods Src expression and purification Recombinant Src and variants were expressed in BL21-AI Escherichia coli (Thermo) containing the pEX-Src-C-His (Origene, Blue Heron Biotech) and purified in a modified procedure as previously described60 (see Supplementary methods for complete details). Mass spectrometry Recombinant Src (~20 µg in dimedone
A Study of the Activity of Recombinant Mn-Superoxide Dismutase in the Presence of Gold and Silver Nanoparticles
Pudlarz, Ranoszek-Soliwoda, Czechowska et al
Appl. Biochem. Biotechnol. (2018)
Pudlarz, Ranoszek-Soliwoda, Czechowska et al
Appl. Biochem. Biotechnol. (2018)
AI Summary: OriGene's pEX-C-His plasmid was used as a cloning vector to express recombinant human Mn-superoxide dismutase with a His-tag, enabling efficient protein purification for the study.
Snippet: reverse), given in italics. The resulting amplified DNA fragments (690 bp) were digested with appropriate restriction enzymes (HindIII and MluI) and cloned into pEX-C-His (OriGene) under T7 promoter. The resulting plasmid
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