PS100083 | pEX-C-GST Bacterial Expression Vector Quimigen Portugal

pEX-C-GST Bacterial Expression Vector

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SKU

PS100083

pEX-C-GST, bacterial expression vector with C-terminal GST tag, 10ug

Specifications
Product Data
C-Tag	GST
E. coli Selection	Ampicillin (100 ug/mL)
Features	This vector is for E.coli expression. Upon IPTG induction, ORFs cloned in this vector can be expressed in E.coli (BL21/DE3) as tagged protein with a C-terminal GST tag. Unlike other PrecisionShuttle vectors, this vector does not have an Sgf I site at the multiple cloning site. Instead a BseR I site can be used to generate Sgf I-compatible end for in-frame shuttling from TrueORF clones in pEntry vector. Detection of the transgene is best done using an anti-GST antibody. If the C-terminal tagging interferes with the protein's function, you can choose the N-terminal GST-tagging vector, PS100029
Download Vector Sequence
Schematic of the multiple cloning sites
Shipping	Blue Ice

Product Manuals
TrueORF and PrecisionShuttle cloning vectors Application Guide
FAQs
FAQ for Tagged Cloning Vectors
SDS for PS100083

Vector Resources
Video Tutorial: Clone Selection Guide Product Video: Why Should You Choose TrueORF GOLD shRNA Selection Guide Protocol: Lentivirus packaging CRISPR Knockout kit manual CRISPR vector manual CRISPR Trangene Knockin at AAVS1 and ROSA26 loci manual CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi) manual AAV Biosafety

Citations

CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome
Asamitsu, Yabuki, Ikenoshita et al
Sci Adv (2021) 7 (3) DOI: 10.1126/sciadv.abd9440

Cited by 104

AI Summary: In this study, OriGene’s pCMV6-Entry-FMR1 plasmid was used to express FMRpolyG protein, enabling the investigation of its interaction with CGG repeat RNA G-quadruplexes and its role in neuronal dysfunction in fragile X-related tremor/ataxia syndrome.

Structural Differences in Translation Initiation between Pathogenic Trypanosomatids and Their Mammalian Hosts
Bochler, Querido, Prilepskaja et al
Cell Rep (2020) 33 (12), 108534 DOI: 10.1016/j.celrep.2020.108534

Cited by 38

AI Summary: OriGene’s pEX-C-GST plasmid (Cat#PS100083) was used in this study to generate GST fusion proteins for pull-down assays, supporting the investigation of structural differences in translation initiation between trypanosomatids and mammalian hosts.

Snippet: pGL4-CMV Wagner et al., 2014 N/A pFASTBAC1-eIF3k Fraser et al., 2004 N/A pFASTBAC1-eIF3d Fraser et al., 2004 N/A pFASTBAC1-eIF3e Fraser et al., 2004 N/A pEX-C-GST OriGene Cat#PS100083 ------------------------- Oligonucleotides ------------------------- Primers for cloning used in this study, see List of primers (Supplemental Information) This paper N;pEX-C-GST OriGene Cat#PS100083

Showing 1-2 of 2 papers.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

pEX-C-GST Bacterial Expression Vector

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pEX-C-GST Bacterial Expression Vector

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pEX-C-GST Bacterial Expression Vector

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pEX-C-GST Bacterial Expression Vector

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