PS100029 | pEX-N-GST Bacterial Expression Vector Quimigen Portugal

pEX-N-GST Bacterial Expression Vector

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PS100029

pEX-N-GST, bacterial expression vector with N-terminal GST tag, 10ug

Specifications
Product Data
N-Tag	GST
E. coli Selection	Ampicillin (100 ug/mL)
Features	Upon IPTG induction, ORFs cloned in this vector can be expressed in E.coli (BL21/DE3) as a tagged protein with an N-terminal GST tag. Detection and purification of the transgene are best done using a glutathione affinity column. If the N-terminal tagging interferes with the protein's function, you can choose the C-terminal GST-tagging vector, PS100083 PS100028_F Primer can be used to sequence the target sequence after cloning. PS100028_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
Shipping	Blue Ice

Product Manuals
TrueORF and PrecisionShuttle cloning vectors Application Guide
FAQs
FAQ for Tagged Cloning Vectors
SDS for PS100029

Vector Resources
Video Tutorial: Clone Selection Guide Product Video: Why Should You Choose TrueORF GOLD shRNA Selection Guide Protocol: Lentivirus packaging CRISPR Knockout kit manual CRISPR vector manual CRISPR Trangene Knockin at AAVS1 and ROSA26 loci manual CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi) manual AAV Biosafety

Citations

The Potential Role of Human NME1 in Neuronal Differentiation of Porcine Mesenchymal Stem Cells: Application of NB-hNME1 as a Human NME1 Suppressor
Cho, Ju, Seo et al
Int J Mol Sci (2021) 22 (22) DOI: 10.3390/ijms222212194

Cited by 3

AI Summary: OriGene's pEX-N-GST vector was used to clone hNME1 and related domains with an N-terminal GST tag, enabling the study of their interactions and functions in neuronal differentiation of porcine mesenchymal stem cells.

Snippet: and domains thereof were cloned along with a N-terminal GST tag into the pEX-N-GST vector (OriGene, Rockville, MD,

Characterisation of three novel β-1,3 glucanases from the medically important house dust mite Dermatophagoides pteronyssinus (airmid)
Waldron, McGowan, Gordon et al
Insect Biochem. Mol. Biol. (2019) 115, 103242 DOI: 10.1016/j.ibmb.2019.103242

AI Summary: OriGene's pEX-N-GST Precision Shuttle vector was used to clone gene fragments encoding the novel β-1,3 glucanases, enabling their recombinant expression and purification for biochemical characterization in this study.

Snippet: S3 Data). Gene fragments were cloned into the pEX-N-GST Precision Shuttle vector (OriGene), recombinantly expressed in and purified from E. coli according to methods described by Dolan et al. (2014). Recombinant proteins

HSP90 promotes cell glycolysis, proliferation and inhibits apoptosis by regulating PKM2 abundance via Thr-328 phosphorylation in hepatocellular carcinoma
Xu, Tu, Dou et al
Mol. Cancer (2017) 16 (1), 178 DOI: 10.1186/s12943-017-0748-y

AI Summary: OriGene’s GST-HSP90 protein was used in GST-pulldown assays to investigate and confirm the interaction between HSP90 and PKM2 in hepatocellular carcinoma cells.

Snippet: GST-HSP90 or control GST (OriGene Technologies, Inc., Rockville, MD, USA) was added into the cell lysates harvested from the cells transfected with HA-tagged PKM2. After being incubated with Glutathione beads (Sigma-Aldrich, St Cached

Structural, mechanistic and functional insight into gliotoxin bis-thiomethylation in Aspergillus fumigatus
Dolan, Bock, Hering et al
Open Biol (2017) 7 (2) DOI: 10.1098/rsob.160292

AI Summary: OriGene’s pEX-N-GST-GtmA expression plasmid was used to produce recombinant GtmA protein in E. coli, enabling the structural and functional studies described in the paper.

Snippet: Expression plasmids pEX-N-GST-GtmA (OriGene) and pET19 m-GtmA (modified pET19b, Novagen) were freshly transformed into chemically competent Escherichia coli BL21 (DE3) cells and subsequently used for pre-culture inoculation in lysogenic broth (LB) supplemented with 100 mg l 2 1 ampicil- lin. Large-scale production of the native protein was carried out in terrific broth including the required ...

A Mutation in NPAS3 That Segregates with Schizophrenia in a Small Family Leads to Protein Aggregation
Nucifora, Wu, Lee et al
Mol Neuropsychiatry (2016) 2 (3), 133-144 DOI: 10.1159/000447358

Cited by 28

AI Summary: OriGene products, specifically the NPAS3 cDNA clone (OriGene, SC119515) and anti-NPAS3 antibody (OriGene, TA324113), were used in this study to express and detect NPAS3 protein variants, enabling the investigation of how the V304I mutation promotes protein aggregation associated with schizophrenia.

Single particle fluorescence burst analysis of epsin induced membrane fission
Brooks, Shoup, Kustigian et al
PLoS ONE (2015) 10 (3), e0119563 DOI: 10.1371/journal.pone.0119563

Cited by 8

AI Summary: OriGene’s pEX-N-His6-GST vector was used to sub-clone and express full-length rat epsin in E. coli, enabling the study of epsin-induced membrane fission in this paper.

Snippet: DTT. The coding sequence of full-length rat epsin was obtained from Addgene (pCDNA3.1-Epsin1;plasmid 22225) and was sub-cloned into the pEX-N-His 6 -GST vector (Origene) for expression in E. coli BL21[DE3]. Purification ... Cite SaveSaving

Showing 1-6 of 9 papers.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

pEX-N-GST Bacterial Expression Vector

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pEX-N-GST Bacterial Expression Vector

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pEX-N-GST Bacterial Expression Vector

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