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> pEX-C-His Bacterial Expression Vector

pEX-C-His Bacterial Expression Vector

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Cat# PS100076

Size : 10ug

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pEX-C-His Bacterial Expression Vector

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SKU
PS100076
pEX-C-His, bacterial expression vector with C-terminal His tag, 10ug
 
Specifications
Product Data
C-Tag His
E. coli Selection Ampicillin (100 ug/mL)
Features

This vector is for E.coli expression.

  • Upon IPTG induction, ORFs cloned in this vector can be expressed in E.coli (BL21/DE3) as tagged protein with a C-terminal His tag.
  • Unlike other PrecisionShuttle vectors, this vector does not have an Sgf I site at the multiple cloning site. Instead a BseR I site can be used to generate Sgf I-compatible end for in-frame shuttling from TrueORF clones in pEntry vector.
  • Detection and purification of the transgene are best done using an anti-His antibody or a nickel affinity column.
  • If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal His-tagging vector, PS100030
Download Vector Sequence
Schematic of the multiple cloning sites
Shipping Blue Ice

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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