Reagents and instruments for immunology, cell biology and molecular biology.
 
Two-step IHC Polymer-based detection kits

Two-step IHC Polymer-based detection kits

wo-step polymer detection kits provide enhanced sensitivity and flexibility for immunohistochemistry (IHC) in rat tissues. They are ideal for detecting low-abundance antigens where background staining or limited signal can affect results.

Principle

The system uses a primary antibody (user-supplied) followed by a polymer-conjugated secondary antibody carrying multiple enzyme molecules (HRP or AP). This two-step amplification improves signal intensity while minimizing non-specific staining.

Advantages

  • High sensitivity: amplifies weak antigen signals for clearer visualization.

  • Species-specific adsorption: secondary antibodies can be adsorbed against rat, rabbit, or human serum to reduce cross-reactivity.

  • Versatile sample compatibility: works with frozen sections, paraffin-embedded tissues, or cell smears.

  • Flexible detection: available in HRP/DAB or AP chromogens, enabling multiplex staining.

Applications

Widely used in preclinical research, neuroscience, immunology, and toxicologic pathology. Two-step polymer kits are particularly useful when 1-step systems provide insufficient sensitivity or when multiplex detection requires sequential amplification.

 

 

 

 

Frequently Asked Questions (FAQ)

How can I quickly find a two-step polymer-based kits product?

To find a two-step polymer-based kits product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.

Does Quimigen stock several product codes in the two-step polymer-based kits category?

Yes, Quimigen distributes several product references in the two-step polymer-based kits category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.

Can I request a quote for two-step polymer-based kits products?

Yes, you can request a quote for two-step polymer-based kits products from the product page or by contacting Quimigen (info@quimigen.pt). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.

How can I find out if a two-step polymer-based kits product is available to order?

The availability of a two-step polymer-based kits product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact Quimigen with the product code or name of the product you are looking for.

What should I do if I cannot find the two-step polymer-based kits product I am looking for?

If the two-step polymer-based kits product you are looking for does not appear in the catalogue, please contact Quimigen with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.

Are all two-step polymer-based kits products available under the same terms and conditions?

No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.

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