RayBio® Human PDGF-AA ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Human PDGF-AA ELISA Kit
Human PDGF-AA ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 40 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 40 pg/ml - 30000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 20 - 200 fold |
| Accession Number | P04085 |
| Gene Id | 5154 |
| Gene Symbols | PDGFA, PDGF1 |
| Protein Name / Synonyms | Platelet-derived growth factor subunit A (PDGF subunit A) (PDGF-1) (Platelet-derived growth factor A chain) (Platelet-derived growth factor alpha polypeptide) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human PDGF-AA ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified PDGF-AA (PDGFA), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Integrated multi-omics profiling of amniotic fluid identifies predictive biomarkers for fetal growth restriction trajectories
- Year:
- 2026
- Journal Name:
- Annals of Medicine
- PubMed ID:
- 42603261
Forskolin Enhances Urokinase Plasminogen Activator Secretion and Angiogenic Activity of Xeno-Free Cultures of Human Adipose Tissue-Derived Stem Cells
- Species:
- Human
- Sample Type:
- Serum (XSFM xeno-free serum-free medium)
- Year:
- 2025
- Journal Name:
- Advanced Biology
- PubMed ID:
- 40631559
Combinatorial immunotherapy of N-803 (IL-15 superagonist) and dinutuximab with ex vivo expanded natural killer cells significantly enhances in vitro cytotoxicity against GD2+ pediatric solid tumors and in vivo survival of xenografted immunodeficient NSG mice
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (NK cells with N-803/dinutuximab)
- Year:
- 2021
- Journal Name:
- Journal for Immunotherapy of Cancer
- PubMed ID:
- 34244307
The Effect of Leukocyte- and Platelet-Rich Fibrin on Central and Peripheral Nervous System Neurons—Implications for Biomaterial Applicability
- Species:
- Human
- Sample Type:
- Leukocyte- and Platelet Rich Fibrin (L-PRF)
- Year:
- 2023
- Journal Name:
- International Journal of Molecular Sciences
Tumor specific cytokine pattern explored in renal cell carcinoma
- Species:
- Human
- Sample Type:
- Serum; Tissue Lysate / Homogenate; Urine
- Year:
- 2026
- Journal Name:
- PLoS ONE
- PubMed ID:
- 42809553
3D printing of silk fibroin-based hybrid scaffold treated with platelet rich plasma for bone tissue engineering.
- Species:
- Human
- Sample Type:
- Plasma (prp)
- Year:
- 2019
- Journal Name:
- Bioactive materials
GSDMB is increased in IBD and regulates epithelial restitution/repair independent of pyroptosis
- Species:
- Human
- Year:
- 2022
Potential Anticarcinogenic Effects From Plasma of Older Adults After Exercise Training: An Exploratory Study
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2022
- Journal Name:
- Frontiers in physiology
Human platelet lysate efficiency, stability, and optimal heparin concentration required in culture of mammalian cells
- Species:
- Human
- Sample Type:
- Tissue Lysate / Homogenate (human platelet lysate HPL)
- Year:
- 2020
- Journal Name:
- Blood research
- PubMed ID:
- 32269973
In vivo assessment of guided neural stem cell differentiation in growthfactor immobilized chitosan-based hydrogel scaffolds
- Species:
- Human
- Sample Type:
- Hydrogel (Hydrogels, cell free)
- Year:
- 2014
- Journal Name:
- Biomaterials
