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Human PDGF-BB ELISA Kit
Human PDGF-BB ELISA Kit
RayBio® Human PDGF-BB ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 1 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 1 pg/ml - 400 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Recommended Dilution (Serum/Plasma) | 5 - 50 fold |
| Accession Number | P01127 |
| Gene Id | 5155 |
| Gene Symbols | PDGFB, SIS, PDGF2 |
| Protein Name / Synonyms | Platelet-derived growth factor subunit B (PDGF subunit B) (PDGF-2) (Platelet-derived growth factor B chain) (Platelet-derived growth factor beta polypeptide) (Proto-oncogene c-Sis) (Becaplermin) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
| Sample type | Cell Culture Supernatants / Plasma / Serum, Cell and Tissue Lysates |
Related Products
NexaTag® Human PDGF-BB qIPCR ELISA Kit
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RayPlex® Human PDGF-BB Bead Assay
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human PDGF-BB ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified PDGF-BB (PDGFB), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)
citations
The HCG11/QKI5 Axis Regulates Endothelial Angiogenic Adaptation During Chronic Cerebral Hypoperfusion
- Species:
- Human
- Sample Type:
- Plasma (chronic cerebral hypoperfusion patients)
- Year:
- 2026
- Journal Name:
- CNS Neuroscience & Therapeutics
- PubMed ID:
- 42484275
Semaphorin-4D signaling in recruiting dental stem cells for vascular stabilization
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (HUVEC supernatants)
- Year:
- 2025
- Journal Name:
- Stem Cell Research & Therapy
- PubMed ID:
- 39865283
PDGF-BB as a potential biomarker for early diagnosis of postpartum hemorrhage
- Species:
- Human
- Sample Type:
- Plasma (PPH, PPH+PE, PE, controls)
- Year:
- 2026
- Journal Name:
- Frontiers in global women's health
- PubMed ID:
- 42389024
Novel platelet-rich plasma/ hyaluronic acid lyophilized formulations for wound healing applications
- Species:
- Human
- Sample Type:
- Other / Unspecified (released protein samples from PRP, HA, PRP gels)
- Year:
- 2025
- Journal Name:
- Frontiers in Bioengineering and Biotechnology
- PubMed ID:
- 41000475
Heparin-conjugated injectable hydrogels with sustained releasing capability for promotion of H-type vessels formation and rat femoral bone defects repair
- Species:
- Human
- Sample Type:
- Human Umbilical Vein Endothelial Cells (HUVECs)
- Year:
- 2023
- Journal Name:
- Materials & Design
Helpful to Live Healthier? Intermittent Hypoxic/Ischemic Training Benefits Vascular Homeostasis and Lipid Metabolism with Activating SIRT1 Pathways in Overweight/Obese Individuals
- Species:
- Human
- Sample Type:
- Serum (overweight/obese individuals)
- Year:
- 2024
- Journal Name:
- Obesity facts
- PubMed ID:
- 38185107
Identification of Salivary Biomarkers in Colorectal Cancer by Integrating Olink Proteomics and Metabolomics
- Species:
- Human
- Sample Type:
- Saliva (14 CRC patients, 14 healthy controls)
- Year:
- 2025
- Journal Name:
- Journal of Proteome Research
- PubMed ID:
- 40183281
Combinatorial immunotherapy of N-803 (IL-15 superagonist) and dinutuximab with ex vivo expanded natural killer cells significantly enhances in vitro cytotoxicity against GD2+ pediatric solid tumors and in vivo survival of xenografted immunodeficient NSG mice
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (NK cells with N-803/dinutuximab)
- Year:
- 2021
- Journal Name:
- Journal for Immunotherapy of Cancer
- PubMed ID:
- 34244307
Brain vascular stability relies on PAK2-cilia-PDGF-BB-HSPGs on basolateral side of endothelium
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (cell-free supernatant of HBMECs)
- Year:
- 2026
- Journal Name:
- Life Science Alliance
- PubMed ID:
- 41490797
Cancer Cell-Derived PDGFB Stimulates mTORC1 Activation in Renal Carcinoma
- Species:
- Human
- Sample Type:
- 786-M1A cell, OS-LM1B cell
- Year:
- 2023
- Journal Name:
- International Journal of Molecular Sciences
