pt en es

Reagentes e instrumentos para imunologia, biologia celular e biologia molecular.

cGAMP [849214-04-6]

Imprimir

Referência HY-12512-1mg

Tamanho : 1mg

Contactar o distribuidor local :

Telefone : +1 850 650 7790

Marca : MedChemExpress

Solicitar mais informações

Contactar o distribuidor local :

Telefone : +1 850 650 7790

Description

cGAMP (Cyclic GMP-AMP) functions as an endogenous second messenger in metazoans and triggers interferon production in response to cytosolic DNA. cGAMP activates stimulator of interferon genes (STING), which activates a signaling cascade leading to the production of type I interferons and other immune mediators^[1]^[2]^[3]^[4].

IC₅₀ & Target

Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
H69AR	EC₅₀	4.74 μM Compound: 3',3'-cGAMP	Activation of wild-type human STING expressed in human THP1-Dual cells co-expressing IRF3-activated ISRE assessed as IRF3 reporter activation measured after 7 hrs by luciferase reporter gene assay Activation of wild-type human STING expressed in human THP1-Dual cells co-expressing IRF3-activated ISRE assessed as IRF3 reporter activation measured after 7 hrs by luciferase reporter gene assay	[PMID: 34019405]
H69AR	EC₅₀	58.46 μM Compound: 3',3'-cGAMP	Activation of wild-type human STING expressed in digitonin treated human THP1-Dual cells co-expressing IRF3-activated ISRE assessed as IRF3 reporter activation measured after 30 mins by luciferase reporter gene assay Activation of wild-type human STING expressed in digitonin treated human THP1-Dual cells co-expressing IRF3-activated ISRE assessed as IRF3 reporter activation measured after 30 mins by luciferase reporter gene assay	[PMID: 34019405]
L929	EC₅₀	20 nM Compound: 3',3'-cGAMP	Inhibition of STING in mouse L929 cells assessed as reduction in cGAMP induce IFNbeta expression measured after 4 hrs by qRT-PCR Inhibition of STING in mouse L929 cells assessed as reduction in cGAMP induce IFNbeta expression measured after 4 hrs by qRT-PCR	[PMID: 32206239]
PBMC	EC₅₀	>300 μM Compound: 3',3'-cGAMP	Induction of TNF-alpha production in healthy human PBMC incubated for 16 hrs Induction of TNF-alpha production in healthy human PBMC incubated for 16 hrs	[PMID: 34019405]
PBMC	EC₅₀	15.14 μM Compound: 3',3'-cGAMP	Induction of INF-gamma production in healthy human PBMC incubated for 16 hrs Induction of INF-gamma production in healthy human PBMC incubated for 16 hrs	[PMID: 34019405]
PBMC	EC₅₀	17.25 μM Compound: 3'3'-cGAMP	Induction of IFNgamma secretion in human PBMC incubated for 16 hrs Induction of IFNgamma secretion in human PBMC incubated for 16 hrs	[PMID: 36201304]
PBMC	EC₅₀	18.72 μM Compound: 3'3'-cGAMP	Induction of TNFalpha secretion in human PBMC incubated for 16 hrs Induction of TNFalpha secretion in human PBMC incubated for 16 hrs	[PMID: 36201304]
PBMC	EC₅₀	26.71 μM Compound: 3',3'-cGAMP	Induction of INF-alpha production in healthy human PBMC incubated for 16 hrs Induction of INF-alpha production in healthy human PBMC incubated for 16 hrs	[PMID: 34019405]
PBMC	EC₅₀	7.65 μM Compound: 3'3'-cGAMP	Induction of IFNalpha secretion in human PBMC incubated for 16 hrs Induction of IFNalpha secretion in human PBMC incubated for 16 hrs	[PMID: 36201304]

In Vitro

cGAMP promotes the antigen-specific proliferation capacity of spleen cells in mice^[2].
cGAMP directly activates murine and human dendritic cells in vitro^[2].
On stimulation with cGAMP, fibroblasts from the patients showed increased transcription of IFNB1 but not of the genes encoding interleukin-1 (IL1), interleukin-6 (IL6), or tumor necrosis factor (TNF)^[3].
cGAMP activates the endoplasmic reticulum (ER)-resident receptor STING, thereby inducing an antiviral state and the secretion of type I IFNs^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

cGAMP (5 μg; nostril mucosal adjuvant) promotes the antigen-specific cytokine production by spleen cells of immunized mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female C57BL/6 (H-2b) mice 6–8 weeks old^[2]
Dosage:	5 µg
Administration:	Nostril mucosal adjuvant
Result:	Higher titers of ovalbumin (OVA)-specific IgA and total IgG as well as IgG1 and IgG2c in the sera of mice immunized with cGAMP-adjuvanted OVA as compared to sera from OVA-immunized mice.

Masse moléculaire

674.41

Formule

C₂₀H₂₄N₁₀O₁₃P₂

CAS No.

849214-04-6

Appearance

Solid

Color

White to yellow

SMILES

O=P(O[C@H]1[C@@H](O)[C@H](N2C=NC3=C2N=CN=C3N)O[C@@H]1COP4(O)=O)(O)OC[C@@H]5[C@@H](O4)[C@@H](O)[C@H](N6C=NC7=C6N=C(N)NC7=O)O5

Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvant et solubilité

In Vitro:

H₂O : 180 mg/mL (266.90 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.4828 mL	7.4139 mL	14.8278 mL
5 mM	0.2966 mL	1.4828 mL	2.9656 mL
10 mM	0.1483 mL	0.7414 mL	1.4828 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Calculateur de molarité
Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Pureté et documentation

Purity: 99.22%

Fiche technique (278 KB) SDS (393 KB)

COA (252 KB) HNMR (258 KB) RP-HPLC (243 KB) MS (127 KB)

Instruction de manipulation (2659 KB)

Références

[1]. Wu J, et al. Cyclic GMP-AMP is an endogenous second messenger in innate immune signaling by cytosolic DNA. Science. 2013 Feb 15;339(6121):826-30. [Content Brief]

[2]. Skrnjug I, et al. Cyclic GMP-AMP displays mucosal adjuvant activity in mice. PLoS One. 2014 Oct 8;9(10):e110150. [Content Brief]

[3]. Liu Y, et al. Activated STING in a vascular and pulmonary syndrome. N Engl J Med. 2014 Aug 7;371(6):507-18. [Content Brief]

[4]. Ablasser A, et al. Cell intrinsic immunity spreads to bystander cells via the intercellular transfer of cGAMP. Nature. 2013 Nov 28;503(7477):530-4. [Content Brief]

Você também pode estar interessado nos seguintes produtos:

Referência

Descrição

Cond.

Price Bef. VAT

ELH-IP10-1

Human IP-10 ELISA Kit

1PlateKit

ELH-IP10-5

Human IP-10 ELISA Kit

5PlateKit

cGAMP [849214-04-6]

Referência HY-12512-1mg

Contactar o distribuidor local :

Marca : MedChemExpress

Contactar o distribuidor local :

cGAMP Related Antibodies

Calculateur de molarité

Calculateur de dilution

Você também pode estar interessado nos seguintes produtos: