RayBio® Human IP-10 (CXCL10) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Human IP-10 ELISA Kit
Human IP-10 ELISA Kit
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 2.5 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 8.23 pg/ml - 6000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP- 2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P02778 |
| Gene Id | 3627 |
| Gene Symbols | CXCL10, INP10, SCYB10 |
| Protein Name / Synonyms | C-X-C motif chemokine 10 (10 kDa interferon gamma-induced protein) (Gamma-IP10) (IP-10) (Small-inducible cytokine B10) [Cleaved into: CXCL10(1-73)] |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Inflammation, Chemokine Biology |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Human IP-10 (CXCL10) qIPCR ELISA Kit
As low as $639.00
RayPlex® Human IP-10 Bead Assay
As low as $240.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human IP-10 ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified IP-10 (CXCL10), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Brexanolone therapeutics in post-partum depression involves inhibition of systemic inflammatory pathways
- Species:
- Human
- Sample Type:
- blood cell
- Year:
- 2023
- Journal Name:
- EBioMedicine
Neural organoids incorporating microglia to assess neuroinflammation and toxicities induced by known developmental neurotoxins
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (cell culture supernatants from neural organoids)
- Year:
- 2025
- Journal Name:
- Current research in toxicology
- PubMed ID:
- 40799410
Sequential Release HydroLipo System for STING Gene Epigenetic Reprogramming and Immune Activation in Glioblastoma
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant
- Year:
- 2025
- Journal Name:
- Advanced science (Weinheim, Baden-Württemberg, Germany)
- PubMed ID:
- 39661716
Radiotherapy enhances CXCR3highCD8+ T cell activation through inducing IFNγ-mediated CXCL10 and ICAM-1 expression in lung cancer cells
- Species:
- Human
- Sample Type:
- Peripheral blood mononuclear cells, A549 cell
- Year:
- 2023
- Journal Name:
- Cancer Immunology Immunotherapy
Human induced pluripotent stem cell engineering establishes a humanized mouse platform for pediatric low-grade glioma modeling
- Species:
- Human
- Sample Type:
- human induced pluripotent stem cell (hiPSC)
- Year:
- 2022
- Journal Name:
- Acta neuropathologica communications
HCV Core Protein Induces Chemokine CCL2 and CXCL10 Expression Through NF-κB Signaling Pathway in Macrophages
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (culture supernatants of macrophages)
- Year:
- 2021
- Journal Name:
- Frontiers in Immunology
- PubMed ID:
- 34531848
CXCL10/IP-10 is a Biomarker and Mediator for Kawasaki Disease
- Species:
- Human
- Sample Type:
- Plasma (Kawasaki's Disease patients)
- Year:
- 2015
- Journal Name:
- Circulation Research
- PubMed ID:
- 25605650
STAT3 inhibition reduces toxicity of oncolytic VSV and provides a potentially synergistic combination therapy for hepatocellular carcinoma
- Species:
- Human
- Sample Type:
- Conditioned Media (Carcinoma Cell lines treated with a Stat3 inhibitor or control)
- Year:
- 2015
- Journal Name:
- Cancer Gene Therapy
- PubMed ID:
- 25930184
Dysfunction of brain pericytes in chronic neuroinflammation
- Species:
- Human
- Sample Type:
- Conditioned Media (HIV innoculated brain periocytes)
- Year:
- 2016
- Journal Name:
- Journal of Cerebral Blood Flow and Metabolism
- PubMed ID:
- 26661157
Novel inflammatory markers associated with cognitive performance: Singapore Longitudinal Ageing Studies
- Species:
- Human
- Sample Type:
- Plasma (Cognitive Performance)
- Year:
- 2016
- Journal Name:
- Neurobiology of Aging
- PubMed ID:
- 26923410
