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Rabbit Anti-VAPB Polyclonal Antibody

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HPA013144

VAMP (vesicle-associated membrane protein)-associated protein B and C

Anti-VAPB Antibody

Polyclonal Antibody against HUMAN VAPB

0.3 mg/ml

info@quimigen.pt

Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

Target protein: VAMP (vesicle-associated membrane protein)-associated protein B and C
Target gene: VAPB
Verified species reactivity: Human, Mouse, Rat
Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000054455 (82%)RAT - ENSRNOG00000005331 (79%)
Clonality: Polyclonal
Isotype: IgG
Host: Rabbit
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet
Purification method: Affinity purified using the PrEST antigen as affinity ligand
Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
- Working concentration: 0.25-2 µg/ml
- Fixation/Permeabilization: PFA/Triton X-100
Immunohistochemistry (IHC) recommended conditions
- Dilution: 1:500 - 1:1000
- Retrieval method: HIER pH6
Western Blot (WB) recommended conditions
- Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)
Immunohistochemistry (IHC)
Western Blot (WB)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues. Validated in Western blot using relevant lysates (see Western blot application images above)

Protein name: VAMP (vesicle-associated membrane protein)-associated protein B and C
Gene name: VAPB
Alternative gene names: ALS8, VAP-B, VAP-C,
Ensembl:
Entrez:
UniProt:

Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

De Tito S, Almacellas E, Yu DD, Millard E, Zhang W, de Heus C, Queval C, Hervás JH, Pellegrino E, Panagi I, Fogde D, Thurston TL, Klumperman J, Gutierrez M, Tooze SADev Cell , . Epub 2025 May 272025 May 27
Li X, Gamuyao R, Wu ML, Cho WJ, Kurtz NB, King SV, Petersen RA, Stabley DR, Lindow C, Climer L, Shirinifard A, Ferrara F, Throm RE, Robinson CG, Carisey A, Tebo AG, Chang CLbioRxiv , . Epub 2023 Nov 292023 Nov 29