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Rabbit Anti-VAPA Polyclonal Antibody

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HPA009174

VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa

Anti-VAPA Antibody

Polyclonal Antibody against HUMAN VAPA

0.1 mg/ml

info@quimigen.pt

Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.

Target protein: VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
Target gene: VAPA
Verified species reactivity: Human
Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000024091 (60%)RAT - ENSRNOG00000014765 (84%)
Clonality: Polyclonal
Isotype: IgG
Host: Rabbit
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet
Purification method: Affinity purified using the PrEST antigen as affinity ligand
Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
- Working concentration: 0.25-2 µg/ml
- Fixation/Permeabilization: PFA/Triton X-100
Immunohistochemistry (IHC) recommended conditions
- Dilution: 1:200 - 1:500
- Retrieval method: HIER pH6
Western Blot (WB) recommended conditions
- Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)
Immunohistochemistry (IHC)
Western Blot (WB)Genetic validation in WB by siRNA knockdown. Validated in Western blot using relevant lysates (see Western blot application images above)

Protein name: VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
Gene name: VAPA
Alternative gene names: hVAP-33, VAP-A,
Ensembl:
Entrez:
UniProt:

Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Li X, Gamuyao R, Wu ML, Cho WJ, Kurtz NB, King SV, Petersen RA, Stabley DR, Lindow C, Climer L, Shirinifard A, Ferrara F, Throm RE, Robinson CG, Carisey A, Tebo AG, Chang CLbioRxiv , . Epub 2023 Nov 292023 Nov 29
Barends M, Koller N, Schölz C, Durán V, Bošnjak B, Becker J, Döring M, Blees H, Förster R, Kalinke U, Tampé RProc Natl Acad Sci U S A , 2023 Jun 12; 120(25):e2219790120. Epub 2023 Jun 122023 Jun 12