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> Human B2M ELISA Kit

Human B2M ELISA Kit

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Referencia ELH-B2M-1

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Marca : RayBiotech

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Human B2M ELISA KitHuman B2M ELISA KitHuman B2M ELISA KitHuman B2M ELISA KitHuman B2M ELISA KitHuman B2M ELISA Kit5 Plate Kit Packaging (one box)

Human B2M ELISA Kit

Human B2M ELISA Kit

RayBio® Human Beta-2 Microglobulin ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Contact Us for Bulk Request

Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit
SpeciesHuman
Sensitivity
6 pg/ml

Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology.

Detection Range
6 pg/ml - 1000 pg/ml
Specificity
Cross Reactivity: This ELISA kit shows no cross-reactivity with any of the following cytokines tested: human Adiponectin (ACRP30), Adipsin (Complement Factor D), Alpha-fetoprotein, ANGPTL4, BCAM, CA125, CA15-3, CEA, CRP (C-Reactive Protein), ErbB2, Ferritin, FSH, GRO alpha (CXCL1), HCG beta, IGF-1 R, IL-1 R2, IL-3, IL-18 R beta (AcPL), IL-21, Leptin, MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, MMP-10, MMP-13, NCAM-1 (CD56), Nidogen-1, NSE, Oncostatin M, Procalcitonin, Prolactin, PSA-free, Siglec-9, TACE, Thyroglobulin, TIMP-4, TSH.
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum
Recommended Dilution (Serum/Plasma)
10,000 - 100,000 fold
Accession Number
P61769
Gene Id
567
Gene Symbols
B2M, CDABP0092, HDCMA22P
Protein Name / Synonyms
Beta-2-microglobulin [Cleaved into: Beta-2-microglobulin form pI 5.3]
Quantitative/Semi-QuantitativeQuantitative
Solid Support
96-well Microplate
Design PrincipleSandwich-based
Method Of DetectionColorimetric
Shipping TypeBlue ice
Storage≤-20°C

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Customize This Human B2M ELISA

We manufacture our ELISAs in-house, so we can build a custom version for your study.

  • Post-translational modifications (PTMs): Detect modified Beta-2 Microglobulin (B2M), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
  • Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
  • Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist

Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.

Learn more about custom ELISA development

documents

Datasheet (ELH-B2M.pdf)
SDS (ELH-B2M.pdf)
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)

citations

  • Plasma Mucin-1 as a Potential Biomarker for Diabetic Peripheral Neuropathy in Type 2 Diabetes

    Species:
    Human
    Sample Type:
    Plasma (T2D with/without peripheral neuropathy)
    Year:
    2026
    Journal Name:
    Biomolecules
    PubMed ID:
    41594668

  • Evaluation of beta-2 microglobulin and alpha-2 macroglobulin levels in patients with different periodontal diseases

    Species:
    Human
    Sample Type:
    Gingival Crevicular Fluid (gingival crevicular fluid)
    Year:
    2013
    PubMed ID:
    23713636

  • Urinary biomarkers of chronic allograft nephropathy

    Species:
    Human
    Sample Type:
    Urine (Renal transplant patients undergoing chronic nephropathy)
    Year:
    2015
    Journal Name:
    Proteomics - Clinical Applications
    PubMed ID:
    25951805

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