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> Human TNF alpha ELISA Kit

Human TNF alpha ELISA Kit

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Human TNF alpha ELISA Kit Standard CurveHuman TNF alpha ELISA KitHuman TNF alpha ELISA KitHuman TNF alpha ELISA KitHuman TNF alpha ELISA KitHuman TNF alpha ELISA KitELH-TNFa was used to measure plasma TNF levels in patients with axial spondyloarthritis and healthy controls. Figure adapted from Prokopcova et al., Heliyon, 2024.ELH-TNFa was used to measure TNF-α levels in lung tissue from immuno-humanized NCG mice following aerosol Mycobacterium tuberculosis infection. Figure adapted from Wang et al., EMBO Molecular Medicine, 2024.ELH-TNFa was used to measure soluble TNF levels in conditioned media from AZD5582-sensitive and -resistant oral squamous cell carcinoma cell lines. Figure adapted from Chai et al., Cancer Research Communications, 2024.ELH-TNFa-CL was used to measure TNF-α levels in whole blood cell lysates from post-partum depression patients before and after brexanolone infusion. Figure adapted from Balan et al., eBioMedicine, 2023.ELH-TNFa was used to measure TNF-α secretion in culture supernatants from cytotoxic T lymphocytes activated by CD47 CAR-macrophages in a SKOV3 ovarian cancer model. Figure adapted from Chen et al., Journal of Translational Medicine, 2023.ELH-TNFa was used to measure TNF-α levels in culture supernatants from human umbilical vein endothelial cells exposed to silica nanoparticles. Figure adapted from Duan et al., International Journal of Nanomedicine, 2014.ELH-TNFa was used to measure TNF-α levels in serum-derived extracellular vesicles from colorectal cancer patients and healthy donors. Figure adapted from Xie et al., Archives of Biochemistry and Biophysics, 2023.Human TNF alpha ELISA KitHuman TNF alpha ELISA KitHuman TNF alpha ELISA Kit5 Plate Kit Packaging (one box)

Human TNF alpha ELISA Kit Standard Curve

Human TNF alpha ELISA Kit

RayBio® Human TNF alpha ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples. 30 pg/ml sensitivity.
Contact Us for Bulk Request

Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit
SpeciesHuman
Sensitivity
30 pg/ml

Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology.

Detection Range
30 pg/ml - 6000 pg/ml
Specificity
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO-alpha, GRO-beta, GRO-gamma, I-309, IFN-gamma, IL-1 alpha, IL-1 beta, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-15, IL-16, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, MCSF, MIG, MIP-1 alpha, MIP-1 beta, NAP-2, PDGF, PF-4.
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Recommended Dilution (Serum/Plasma)
2 fold
Accession Number
P01375
Gene Id
7124
Gene Symbols
TNF, TNFA, TNFSF2
Protein Name / Synonyms
Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor, membrane form (N-terminal fragment) (NTF) Intracellular domain 1 (ICD1) Intracellular domain 2 (ICD2) C-domain 1 C-domain 2 Tumor necrosis factor, soluble form]
Quantitative/Semi-QuantitativeQuantitative
Solid Support
96-well Microplate
Design PrincipleSandwich-based
Method Of DetectionColorimetric
Research AreaCardiovascular Disease, Cancer, Inflammation, Antibody Drug Development, Protein Therapeutics
Shipping TypeBlue ice
Storage≤-20°C

Target Background

Tumor necrosis factor alpha (TNF alpha), also known as lymphotoxin and cachectin, is a pleiotropic cytokine. TNF alpha is a smaller cytokine with a molecular weight of 26 kDa (transmembrane) and 17 kDa (soluble) that binds to the same receptors producing a vast array of effects similar to those of TNF-beta. Both TNF-alpha and TNF-beta share 30% amino acid homology and have similar biological activities. TNF-alpha is produced primarily by monocytes, neutrophils and macrophages. It is found in synovial cells and macrophages in the tissues. TNF-alpha and TNF-beta can modulate many immune and inflammatory functions, while having the ability to inhibit tumor growth. Target tumor cells must express TNF receptors 1 (55 kDa) and 2 (75 kDa) to be killed, with the p55 receptor mediating the cytotoxic response.

Product Features

  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Standard curve graphs showing the results from the human tnf alpha elisa kit

Dilution series diagram with 8 vials

Spiking & Recovery Results

Sample TypeAverage % RecoveryRange (%)
Serum94.5382-103
Plasma91.9280-102
Cell culture media96.4384-104

Linearity Results

  SerumPlasmaCell Culture Media
1:2Average % of expected
Range (%)		94
83-102		93
82-103		92
81-102
1:4Average % of expected
Range (%)		94
83-104		92
82-103		99
85-107

Typical Data

ELH-TNFa was used to measure plasma TNF levels in patients with axial spondyloarthritis and healthy controls. Figure adapted from Prokopcova et al., Heliyon, 2024.

ELH-TNFa was used to measure TNF-α levels in lung tissue from immuno-humanized NCG mice following aerosol Mycobacterium tuberculosis infection. Figure adapted from Wang et al., EMBO Molecular Medicine, 2024.

ELH-TNFa was used to measure soluble TNF levels in conditioned media from AZD5582-sensitive and -resistant oral squamous cell carcinoma cell lines. Figure adapted from Chai et al., Cancer Research Communications, 2024.

ELH-TNFa-CL-1 was used to measure TNF-α levels in whole blood cell lysates from post-partum depression patients before and after brexanolone infusion. Figure adapted from Balan et al., eBioMedicine, 2023.

ELH-TNFa was used to measure TNF-α secretion in culture supernatants from cytotoxic T lymphocytes activated by CD47 CAR-macrophages in a SKOV3 ovarian cancer model. Figure adapted from Chen et al., Journal of Translational Medicine, 2023.

ELH-TNFa was used to measure TNF-α levels in culture supernatants from human umbilical vein endothelial cells exposed to silica nanoparticles. Figure adapted from Duan et al., International Journal of Nanomedicine, 2014.

ELH-TNFa was used to measure TNF-α levels in serum-derived extracellular vesicles from colorectal cancer patients and healthy donors. Figure adapted from Xie et al., Archives of Biochemistry and Biophysics, 2023.

Kit Components

  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate

Additional Materials Required

  • Distilled or deionized water
  • Precision pipettes to deliver 2 µL to 1 µL volumes
  • Adjustable 1-25 µL pipettes for reagent preparation
  • 100 µL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Assay Procedure Summary

  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µL of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Customize This Human TNF alpha ELISA

We manufacture our ELISAs in-house, so we can build a custom version for your study.

  • Post-translational modifications (PTMs): Detect modified TNF alpha (TNFA), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
  • Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
  • Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist

Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.

Learn more about custom ELISA development

documents

Datasheet (ELH-TNFa.pdf)
Datasheet (ELH-TNFa-CL.pdf)
SDS (ELH-TNFa.pdf)
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)

citations

  • Enhanced sialylation of bone marrow mesenchymal stromal cells contributes to immune remodeling through macrophage polarization in multiple myeloma patients

    Species:
    Human
    Sample Type:
    Cell Culture Supernatant (culture supernatants of primary macrophages)
    Year:
    2026
    Journal Name:
    Frontiers in Immunology
    PubMed ID:
    42712628

  • The HCG11/QKI5 Axis Regulates Endothelial Angiogenic Adaptation During Chronic Cerebral Hypoperfusion

    Species:
    Human
    Sample Type:
    Plasma (chronic cerebral hypoperfusion patients)
    Year:
    2026
    Journal Name:
    CNS Neuroscience & Therapeutics
    PubMed ID:
    42484275

  • GPRC5A/CXCL8/NLRP3-mediated neutrophil extracellular traps drive gemcitabine-nab-paclitaxel resistance in pancreatic adenocarcinoma

    Species:
    Human
    Sample Type:
    Cell Culture Supernatant (cell culture supernatants of GnP-resistant MIA PaCa-2 or PANC-1 cells)
    Year:
    2025
    Journal Name:
    Cancer Biology & Medicine
    PubMed ID:
    40665634

  • A 3D Alginate-Gelatin Co-Culture Model to Study Epithelial-Stromal Interactions in the Gut

    Species:
    Human
    Sample Type:
    Cell Culture Supernatant (HT-29/IMR-90 3D co-culture)
    Year:
    2026
    Journal Name:
    Gels (Basel, Switzerland)
    PubMed ID:
    41590095

  • Adjunctive effect of gaseous ozone on inflammatory biomarkers and microbial profile in grade C molar-incisor pattern periodontitis: a randomized split-mouth placebo-controlled clinical trial

    Species:
    Human
    Sample Type:
    Other Body Fluid (sample strips eluate gingival crevicular fluid)
    Year:
    2026
    Journal Name:
    BMC oral health
    PubMed ID:
    41942979

  • Elevated Salivary TNF-α Levels and Clinical Severity of Oral Lichen Planus

    Species:
    Human
    Sample Type:
    Saliva (unstimulated whole saliva)
    Year:
    2026
    Journal Name:
    Dentistry 3000

  • Sleep disturbances, inflammation, and muscle function in older adults with sarcopenia

    Species:
    Human
    Sample Type:
    Whole Blood (older adults with sarcopenia)
    Year:
    2026
    Journal Name:
    Sleep & breathing = Schlaf & Atmung
    PubMed ID:
    42696225

  • Spent Hop (Humulus lupulus L.) Extract and Its Flaxseed Polysaccharide-Based Encapsulates Attenuate Inflammatory Bowel Diseases Through the Nuclear Factor-Kappa B, Extracellular Signal-Regulated Kinase, and Protein Kinase B Signalling Pathways

    Species:
    Human
    Sample Type:
    Cell Lysate (protein cell lysates); Tissue Lysate / Homogenate
    Year:
    2025
    Journal Name:
    Cells
    PubMed ID:
    40710352

  • Late-pregnancy air pollution exposure and neonatal anthropometry with and without gestational hypertension

    Species:
    Human
    Sample Type:
    Serum (maternal serum); Whole Blood (umbilical cord blood)
    Year:
    2026
    Journal Name:
    iScience
    PubMed ID:
    42602318

  • Effects of Acute Fish Oil Supplementation on Muscle Function and Soreness After Eccentric Contraction-Induced Muscle Damage

    Species:
    Human
    Sample Type:
    Plasma (after eccentric muscle damage)
    Year:
    2025
    Journal Name:
    Nutrients
    PubMed ID:
    41228481

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