PerCP-Cy5.5 conjugated anti-bovine primary antibodies are fluorescent immunoreagents used in veterinary and comparative immunology to detect bovine antigens in multicolor flow cytometry. They enable far-red emission detection (~695 nm), supporting high-resolution immune profiling with minimal spectral overlap.
Fluorophore Properties
PerCP-Cy5.5 is a tandem dye derived from PerCP (excitation ~490 nm) transferring energy to Cy5.5 emission around 695 nm, offering improved photostability, low autofluorescence, and bright signal intensity suitable for fixed bovine tissue assays.
Antibody Conjugation and Targets
Antibodies are typically mouse or rabbit IgG conjugated via amine-reactive chemistry, targeting bovine CD markers, immunoglobulins, cytokines, or pathogen proteins, maintaining high affinity and optimized fluorophore-to-protein ratios for reliable quantification.
Applications
Applications include flow cytometry of bovine PBMCs for immune profiling, immunofluorescence imaging of infected tissues, and high-throughput screening in 96-well plate formats for diseases such as bovine respiratory infections. These tools support vaccine development and diagnostics in cattle health research worldwide with reproducible, high-sensitivity readouts across experimental settings consistently.
