PerCP-conjugated anti-Drosophila primary antibodies are affinity-purified monoclonal or polyclonal antibodies directly labeled with Peridinin-Chlorophyll a Protein (PerCP). Excited by the standard 488 nm laser, PerCP emits bright far-red fluorescence (≈675–685 nm), making these antibodies ideal for multicolor flow cytometry while eliminating secondary antibody incubation, reducing background, and improving workflow efficiency.
Key Features
- Far-red fluorescence: Excellent spectral separation for complex multicolor panels with minimal autofluorescence.
- High sensitivity: Bright fluorescence enables reliable detection of low-abundance Drosophila proteins.
- Direct detection: No secondary antibody required, reducing assay time and cross-reactivity.
- Validated performance: Available in monoclonal and polyclonal formats with optimized labeling and lot-specific quality control.
Applications
These antibodies are primarily used for flow cytometry (FACS), multicolor immunophenotyping, bead-based multiplex assays, and fluorescence-activated cell sorting. They also support the detection of developmental, neural, cytoskeletal, signaling, histone, and tagged proteins in Drosophila melanogaster. Although compatible with fluorescence microscopy using appropriate far-red filters, their principal application remains high-performance flow cytometric analysis.
