Low endotoxin anti-bovine primary antibodies are purified to remove bacterial lipopolysaccharides (LPS), ensuring minimal immune activation. These ultra-pure reagents are specifically designed for sensitive applications such as live cell assays and in vivo studies, where endotoxin contamination can compromise experimental outcomes.
Endotoxin Effects
- LPS can activate immune cells at extremely low concentrations, leading to unintended responses.
- Conventional antibodies may contain endotoxin levels sufficient to induce experimental artifacts.
- Low endotoxin formulations remain below activation thresholds, preserving biological accuracy.
Purification and Properties
- Advanced chromatography and filtration remove endotoxin while maintaining antibody integrity.
- Supplied carrier-free in PBS, ensuring compatibility with downstream applications.
- Low aggregation minimizes non-specific binding and improves reproducibility.
Applications
- Functional assays, neutralization studies, and primary bovine cell culture.
- In vivo experiments requiring minimal inflammatory interference.
- Flow cytometry without endotoxin-induced activation artifacts.