Quantitative dolphin cytokine antibody arrays are multiplex immunoassays designed to measure multiple cytokines simultaneously in biological samples from dolphins. Based on a sandwich ELISA principle, these glass-slide protein microarrays use immobilized capture antibodies and biotinylated detection antibodies to quantify selected immune mediators through fluorescence detection. Quantitative arrays use protein standards to generate calibration curves, enabling the estimation of cytokine concentrations in tested samples.

Key Features

• Species-specific profiling: Designed to detect a panel of 10 dolphin cytokines involved in inflammatory and immune responses.

• Multiplex protein detection: Enables simultaneous analysis of multiple cytokine targets on a single glass-slide array.

• Quantitative analysis: Uses target-specific protein standards and calibration curves to estimate cytokine concentrations.

• Multiple sample types: Compatible with serum, plasma, cell culture supernatants, cell lysates and tissue lysates, according to the manufacturer's specifications.

• Fluorescence-based readout: Requires a compatible laser fluorescence scanner and quantitative data analysis.

Usual Research Applications

• Marine mammal immunology: Investigating cytokine profiles associated with immune regulation and inflammatory responses in dolphins.

• Comparative immunology: Exploring cytokine responses across experimental conditions and biological models.

• Environmental and health research: Investigating associations between cytokine profiles and physiological or environmental factors.

• Biomarker discovery: Screening multiple immune mediators to identify candidates for further validation using individual assays.