Biotinylated anti-monkey primary antibodies are immunoreagents in which a biotin molecule is covalently attached to an immunoglobulin directed against non-human primate (NHP) antigens. These reagents are widely employed in indirect detection systems, where the biotin moiety serves as a high-affinity handle for streptavidin- or avidin-conjugated reporters carrying enzymes, fluorophores, or other detection labels. The biotin–streptavidin interaction (Kd ≈ 10⁻¹⁴ M) provides exceptional signal amplification, enabling sensitive detection of monkey immunoglobulins, cell surface markers, and cytokines in ELISA, flow cytometry, Western blotting, immunohistochemistry, and ELISpot assays.
Applications
Biotinylated anti-monkey antibodies are validated for a broad range of immunoassay applications.
ELISA. These conjugates are extensively used as secondary detection reagents in enzyme-linked immunosorbent assays. The anti-Monkey IgG (gamma chain) biotin conjugate is recommended for ELISA at dilutions of 1:20,000 to 1:50,000.
Flow Cytometry and FACS. Biotinylated anti-monkey antibodies are used with streptavidin-fluorophore conjugates for indirect staining of monkey leukocyte subsets. .
Western Blotting. These conjugates are used with streptavidin-HRP and chemiluminescent substrates for detection of monkey proteins.
Immunohistochemistry. Biotinylated anti-monkey antibodies are suitable for immunohistochemical staining of frozen and paraffin-embedded tissue sections.
ELISpot. The Biotinylated Anti-cynomolgus monkey CXCL9 Polyclonal Antibody has been validated for ELISA and ELISpot applications.