Anti-rat primary antibodies are immunoglobulins that bind specific antigens expressed in the rat (Rattus norvegicus), one of the most widely used model organisms in physiology, immunology, neuroscience, and pharmacology. They are produced by immunizing a host species—commonly mouse, rabbit, or goat—with a rat-derived antigen and purifying the resulting immunoglobulin for use in detection, localization, or functional perturbation of the target protein.
Applications
Anti-rat primary antibodies are validated across standard immunoassay platforms. In flow cytometry, they enable immunophenotyping of rat leukocyte subsets, including granulocytes, macrophages, dendritic cells, and natural killer cells, through markers such as CD11b. In immunohistochemistry, they are used on frozen and paraffin-embedded rat tissues for spatial localization of antigens, with clone OX-41 against CD172a (SIRPα) validated for flow cytometry, immunohistochemistry, immunoprecipitation, and Western blotting. In Western blotting, they support detection of rat proteins in tissue and cell lysates, and in immunoprecipitation they enable capture of native rat protein complexes.
Beyond detection, a distinctive application of anti-rat primary antibodies is in vivo functional modulation. Selected antibodies can block receptor activity, modulate signaling pathways, or promote depletion of specific cell populations in rat disease models, depending on target expression, antibody isotype, and Fc-mediated effector functions. This makes them valuable tools for investigating immune-cell populations, inflammatory mechanisms, and cell-surface receptor signaling in vivo.
