Allophycocyanin (APC)-conjugated anti–guinea pig primary antibodies are fluorescent detection reagents used to identify guinea pig immunoglobulins or guinea pig-derived targets in flow cytometry and fluorescence-based assays. APC is a bright far-red phycobiliprotein enabling high sensitivity and compatibility with multicolor panels.
Structure and Properties
These antibodies are typically affinity-purified and may be polyclonal or F(ab')2 formats, optimized for surface staining with minimal cross-reactivity.
They commonly support flow cytometry, ELISA, microarray assays, and other fluorescence-based detection workflows, with excitation around 620–650 nm and emission around 660–670 nm.
Storage conditions generally require 2–8 °C refrigeration, ensuring antibody stability and performance over time.
Applications
APC-conjugated anti–guinea pig antibodies are widely used in flow cytometry for cell-surface phenotyping in multicolor experiments. They are also applied in ELISA and fluorescence-based assays requiring high sensitivity, but are less suitable for deep tissue imaging due to the large size of APC, making them best suited for suspension cell studies.
Overall APC enables robust far-red fluorescence detection in immunoassays applications.
