Azide-free anti-bovine primary antibodies eliminate sodium azide preservative to support live-cell and functional biological applications.
These formulations maintain antibody stability without chemical stabilizers, enabling compatibility with conjugation workflows and cell-based experimental systems.
Purpose and Benefits
Sodium azide is commonly used as a preservative but can interfere with biological activity in live systems and downstream labeling reactions.
- Live-cell compatibility: Enables use in functional assays without cytotoxic effects.
- Conjugation-ready: Suitable for fluorophore, enzyme, and nanoparticle labeling.
- Reduced background: High purity improves signal quality in sensitive detection systems.
Formulation Characteristics
- Supplied in PBS buffer, typically at 0.5–2 mg/mL.
- Available in liquid or lyophilized formats.
- Low endotoxin content for sensitive biological applications.
- Free from BSA and glycerol carriers.
Stability and Storage
- Liquid formulations remain stable for 6–12 months at 4°C.
- Lyophilized forms remain stable for more than 2 years at -20°C.
- Recommended aliquoting to avoid repeated freeze–thaw cycles.
Key Applications
- Live-cell flow cytometry without fixation-induced toxicity.
- Functional blocking and neutralization assays.
- Custom conjugation with dyes, enzymes, and nanoparticles.
- Cell proliferation and cytotoxicity studies.