Atto-conjugated anti-mouse antibodies are fluorescent immunoreagents that combine the photostability and brightness of ATTO-TEC dyes with the target specificity of immunoglobulins directed against mouse antigens. Because the laboratory mouse (Mus musculus) is the most widely used mammalian model organism, reagents enabling sensitive and reproducible detection of mouse proteins are indispensable across immunology, neuroscience, cancer biology, and stem cell research.
Applications
ATTO-conjugated anti-mouse antibodies are validated across a broad spectrum of immunoassay applications:
- Flow cytometry: Immunophenotyping of mouse leukocyte subsets and subclasses, with typical working dilutions of 1:500–1:2,500.
- Immunofluorescence microscopy: Localization of mouse antigens in cultured cells and tissue sections, with working dilutions typically ranging from 1:5,000.
- STED super-resolution microscopy: ATTO dyes are specifically designed for STED microscopy, with ATTO 647N and ATTO 655 particularly well suited for this application.
- FRET: The ATTO dye family is designed for FRET applications, enabling studies of molecular interactions.
- Fluorescence-based plate assays (FLISA): With working dilutions of >1:20,000.
- Fluorescent Western blotting: With working dilutions of >1:10,000.
