Scavenger Receptor A Type I/II (CD204, SRA) (FITC)

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Cat# S0099-06-500ug

Size : 500ug

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Phone : +1 850 650 7790

Brand : US Biological

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Phone : +1 850 650 7790

S0099-06 Scavenger Receptor A Type I/II (CD204, SRA) (FITC)

Clone Type

Monoclonal

Host

rat

Source

mouse

Swiss Prot

P30204

Isotype

IgG2b

Grade

Affinity Purified

Applications

Crossreactivity

Mo Po

Shipping Temp

Blue Ice

Storage Temp

-20°C

CD204 is expressed by tissue macrophages and functions both as an endocytic receptor for lipoproteins and as an adhesion receptor for macrophages binding to ligand rich tissues e.g. atherosclerotic lesions. Inhibits the uptake of acetylated low-density lipoproteins and also inhibits divalent cation independent adhesion.

Applications:
Suitable for use in Flow Cytometry. Not suitable for Immunohistochemistry, paraffin or resin. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 10ul labels 10e6 cells in 100ul.
Optimal dilution determined by the researcher.

Recommended Negative Control:
I1904-84J1: IgG2b Negative Control (FITC)

Hybridoma:
Spleen cells from immunized AO rats were fused with cells of the Y3 rat myeloma cell line.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications

Product Type: Mab|Isotype: IgG2b|Clone No: 6A458 (2F8)|Host: rat|Source: mouse|Concentration: ~0.1mg/ml|Form: Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with Fluorescein Isothiocyanate Isomer 1 (FITC). |Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Immunogen: RAW264 cell line|Specificity: Recognizes mouse scavenger receptor class A (SRA), type I and II, also known as CD204. Recognizes an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice and is therefore unsuitable for use with the C57BL/6 mouse strain. Species Crossreactivity: porcine and channel catfish||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

RAW264 cell line

Form

Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with Fluorescein Isothiocyanate Isomer 1 (FITC).

Purity

Purified by Protein G affinity chromatography from tissue culture supernatant.

Specificity

Recognizes mouse scavenger receptor class A (SRA), type I and II, also known as CD204. Recognizes an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice and is therefore unsuitable for use with the C57BL/6 mouse strain. Species Crossreactivity: porcine and channel catfish

References

1. Fraser, I.P. et al. (1993). Nature 364: 343-346. 2. de Villiers, W.J.S. et al. (1994). J. Exp. Med. 180: 705-709. 3. Hughes, D.A. et al. (1995). . Eur. J. Immunol. 25: 466-473. 4. Bell, M.D. et al. (1994). J. Neurocytol. 23: 605-613. 5. Hughes, D.A. et al. (1994). . Imm. Letts. 43: 7-14. 6 Rosen, H. and Hughes, D.A. (1995). Assays of Myeloid Cell Function: Migration and adhesion in vivo. Weir Handbook of Experimental Immunology. London, Blackwell Scientific Publications. 5th, ed. In Press. 7. Daugherty, A. et al. (2000). Polymorphism of class A scavenger receptors in C57BL/6 mice. J. Lipid Res. 41: 1568 - 1577