Reactivos e instrumentos para inmunología, biología celular y biología molecular.
 
> Scavenger Receptor A Type I/II (CD204, SRA) (FITC)

Scavenger Receptor A Type I/II (CD204, SRA) (FITC)

Imprimir Imprimir
Por favor conéctese

Referencia S0099-06-500ug

embalaje : 500ug

Marca : US Biological

Contact local distributor :


Teléfono : +1 850 650 7790

S0099-06 Scavenger Receptor A Type I/II (CD204, SRA) (FITC)

Clone Type
Monoclonal
Host
rat
Source
mouse
Swiss Prot
P30204
Isotype
IgG2b
Grade
Affinity Purified
Applications
FC
Crossreactivity
Mo Po
Shipping Temp
Blue Ice
Storage Temp
-20°C

CD204 is expressed by tissue macrophages and functions both as an endocytic receptor for lipoproteins and as an adhesion receptor for macrophages binding to ligand rich tissues e.g. atherosclerotic lesions. Inhibits the uptake of acetylated low-density lipoproteins and also inhibits divalent cation independent adhesion.

Applications:
Suitable for use in Flow Cytometry. Not suitable for Immunohistochemistry, paraffin or resin. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 10ul labels 10e6 cells in 100ul.
Optimal dilution determined by the researcher.

Recommended Negative Control:
I1904-84J1: IgG2b Negative Control (FITC)

Hybridoma:
Spleen cells from immunized AO rats were fused with cells of the Y3 rat myeloma cell line.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG2b|Clone No: 6A458 (2F8)|Host: rat|Source: mouse|Concentration: ~0.1mg/ml|Form: Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with Fluorescein Isothiocyanate Isomer 1 (FITC). |Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Immunogen: RAW264 cell line|Specificity: Recognizes mouse scavenger receptor class A (SRA), type I and II, also known as CD204. Recognizes an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice and is therefore unsuitable for use with the C57BL/6 mouse strain. Species Crossreactivity: porcine and channel catfish||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
RAW264 cell line
Form
Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with Fluorescein Isothiocyanate Isomer 1 (FITC).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes mouse scavenger receptor class A (SRA), type I and II, also known as CD204. Recognizes an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice and is therefore unsuitable for use with the C57BL/6 mouse strain. Species Crossreactivity: porcine and channel catfish
References
1. Fraser, I.P. et al. (1993). Nature 364: 343-346. 2. de Villiers, W.J.S. et al. (1994). J. Exp. Med. 180: 705-709. 3. Hughes, D.A. et al. (1995). . Eur. J. Immunol. 25: 466-473. 4. Bell, M.D. et al. (1994). J. Neurocytol. 23: 605-613. 5. Hughes, D.A. et al. (1994). . Imm. Letts. 43: 7-14. 6 Rosen, H. and Hughes, D.A. (1995). Assays of Myeloid Cell Function: Migration and adhesion in vivo. Weir Handbook of Experimental Immunology. London, Blackwell Scientific Publications. 5th, ed. In Press. 7. Daugherty, A. et al. (2000). Polymorphism of class A scavenger receptors in C57BL/6 mice. J. Lipid Res. 41: 1568 - 1577