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> Rabbit Anti-EMD Polyclonal Antibody

Rabbit Anti-EMD Polyclonal Antibody

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Cat# HPA000609

Size : 100ul

Brand : Atlas Antibodies

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Phone : +1 850 650 7790

HPA000609
emerin

Anti-EMD Antibody

Polyclonal Antibody against HUMAN EMD

0.05 mg/ml
Anti-EMD Antibody
Anti-EMD Antibody
Anti-EMD Antibody
Anti-EMD Antibody
Anti-EMD Antibody
Anti-EMD Antibody
Anti-EMD Antibody
Anti-EMD Antibody
Anti-EMD Antibody
Anti-EMD Antibody
Anti-EMD Antibody
Anti-EMD Antibody
Anti-EMD Antibody
Anti-EMD Antibody
Anti-EMD Antibody
info@quimigen.pt

Immunohistochemical staining of human skeletal muscle shows strong positivity in nuclear membrane in myocytes.

Product details

  • Target protein: emerin
  • Target gene: EMD
  • Verified species reactivity: Human, Mouse, Rat
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000001964 (71%)RAT - ENSRNOG00000058882 (73%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: 000068827
  • Concentration: 0.05 mg/ml

Applications

  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:200 - 1:500
    • Retrieval method: HIER pH6
  • Western Blot (WB) recommended conditions
    • Working concentration: 0.04-0.4 µg/ml
  • Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
    • Working concentration: 0.25-2 µg/ml
    • Fixation/Permeabilization: PFA/Triton X-100

Validation

  • Immunohistochemistry (IHC) 
  • Western Blot (WB)Genetic validation in WB by siRNA knockdown. Validated in Western blot using relevant lysates (see Western blot application images above) 
  • Immunofluorescence in Cell Lines (ICC-IF) 

Target information

  • Protein name: emerin
  • Gene name: EMD
  • Alternative gene names: LEMD5, STA, 
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (5)

  • Osmanagic-Myers S, Kiss A, Manakanatas C, Hamza O, Sedlmayer F, Szabo PL, Fischer I, Fichtinger P, Podesser BK, Eriksson M, Foisner RJ Clin Invest , 2018 Dec 18; 129(2):531-545. Epub 2018 Dec 182018 Dec 18
  • Jafferali MH, Figueroa RA, Hasan M, Hallberg ESci Rep , 2017 Nov 30; 7:16655. Epub 2017 Nov 302017 Nov 30

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