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> Mouse Anti-EZR Monoclonal Antibody [Clone:CL2378]

Mouse Anti-EZR Monoclonal Antibody [Clone:CL2378]

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Cat# AMAb90979

Size : 100ul

Brand : Atlas Antibodies

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Phone : +1 850 650 7790

AMAb90979
ezrin

Anti-EZR Antibody

Monoclonal Antibody against HUMAN EZR

1.0 mg/ml
Anti-EZR Antibody
Anti-EZR Antibody
Anti-EZR Antibody
Anti-EZR Antibody
Anti-EZR Antibody
Anti-EZR Antibody
Anti-EZR Antibody
Anti-EZR Antibody
Anti-EZR Antibody
Anti-EZR Antibody
Anti-EZR Antibody
Anti-EZR Antibody
Anti-EZR Antibody
Anti-EZR Antibody
Anti-EZR Antibody
Anti-EZR Antibody
Anti-EZR Antibody
info@quimigen.pt

Immunohistochemistry analysis in human rectum and skeletal muscle tissues using AMAb90979 antibody. Corresponding EZR RNA-seq data are presented for the same tissues.

Product details

  • Target protein: ezrin
  • Target gene: EZR
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000052397 (93%)RAT - ENSRNOG00000018524 (93%)
  • Clonality: Monoclonal
  • Clone ID: CL2378
  • Isotype: IgG2b
  • Host: Mouse
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Protein A purified
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: MAB-02857
  • Concentration: 1.0 mg/ml

Applications

  • Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
    • Working concentration: 2-10 µg/ml
    • Fixation/Permeabilization: PFA/Triton X-100
  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:5000 - 1:10000
    • Retrieval method: HIER pH6
  • Western Blot (WB) recommended conditions
    • Working concentration: 1 µg/ml

Validation

  • Immunofluorescence in Cell Lines (ICC-IF) 
  • Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.  
  • Western Blot (WB)Validated in Western blot using relevant lysates (see Western blot application images above) 

Target information

  • Protein name: ezrin
  • Gene name: EZR
  • Alternative gene names: VIL2, 
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (1)

  • This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
    Human Protein Atlas

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