Rabbit Anti-CRBN Polyclonal Antibody

Print

Cat# HPA045910

Size : 100ul

Contact local distributor :

Phone : +1 850 650 7790

Brand : Atlas Antibodies

Request more information

Contact local distributor :

Phone : +1 850 650 7790

HPA045910

cereblon

Anti-CRBN Antibody

Polyclonal Antibody against HUMAN CRBN

0.2 mg/ml

info@quimigen.pt

Western blot analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-CRBN antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Product details

Target protein: cereblon
Target gene: CRBN
Verified species reactivity: Human, Mouse, Rat
Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000005362 (96%)RAT - ENSRNOG00000006534 (97%)
Clonality: Polyclonal
Isotype: IgG
Host: Rabbit
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet
Purification method: Affinity purified using the PrEST antigen as affinity ligand
Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

Unit size: 100µl
Current lot: 000028938
Concentration: 0.2 mg/ml

Applications

Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
- Working concentration: 0.25-2 µg/ml
- Fixation/Permeabilization: PFA/Triton X-100
Western Blot (WB) recommended conditions
- Working concentration: 0.04-0.4 µg/ml

Validation

Immunofluorescence in Cell Lines (ICC-IF)
Western Blot (WB)Genetic validation in WB by siRNA knockdown. Validated in Western blot using relevant lysates (see Western blot application images above)

Target information

Protein name: cereblon
Gene name: CRBN
Alternative gene names: MRT2, MRT2A,
Ensembl:
Entrez:
UniProt:

Shipping and storage

Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (32)

Guchhait K, Yoon HS, An HS, Shin S, Nam HS, Yanqui-Rivera FD, Oña SM, Mendez MÁ, Hwang JY, Park D, Park CS, Han JY, Chung DY, Park S, Kim EK, Yang SG, Cho SKOncogene , 2026 Apr; 45(14):1234-1246. Epub 2026 Mar 92026 Apr
Huber AD, Lin W, Jung YH, Poudel S, Yang G, Wu J, Carrigan AG, Pagala V, Wang W, Fu Y, Yuan ZF, Byrum SD, Yang K, Gee RR, Arnold ED, Loughran AJ, Wang J, Pruett-Miller SM, Peng J, Chen TNat Commun , 2025 Nov 6; 16:9805. Epub 2025 Nov 62025 Nov 6