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> Rabbit Anti-CRBN Polyclonal Antibody

Rabbit Anti-CRBN Polyclonal Antibody

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Referencia HPA045910

embalaje : 100ul

Marca : Atlas Antibodies

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HPA045910
cereblon

Anti-CRBN Antibody

Polyclonal Antibody against HUMAN CRBN

0.2 mg/ml
Anti-CRBN Antibody
Anti-CRBN Antibody
Anti-CRBN Antibody
Anti-CRBN Antibody
Anti-CRBN Antibody
Anti-CRBN Antibody
Anti-CRBN Antibody
info@quimigen.pt

Western blot analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-CRBN antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Product details

  • Target protein: cereblon
  • Target gene: CRBN
  • Verified species reactivity: Human, Mouse, Rat
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000005362 (96%)RAT - ENSRNOG00000006534 (97%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: 000028938
  • Concentration: 0.2 mg/ml

Applications

  • Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
    • Working concentration: 0.25-2 µg/ml
    • Fixation/Permeabilization: PFA/Triton X-100
  • Western Blot (WB) recommended conditions
    • Working concentration: 0.04-0.4 µg/ml

Validation

  • Immunofluorescence in Cell Lines (ICC-IF) 
  • Western Blot (WB)Genetic validation in WB by siRNA knockdown. Validated in Western blot using relevant lysates (see Western blot application images above) 

Target information

  • Protein name: cereblon
  • Gene name: CRBN
  • Alternative gene names: MRT2, MRT2A, 
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (32)

  • Guchhait K, Yoon HS, An HS, Shin S, Nam HS, Yanqui-Rivera FD, Oña SM, Mendez MÁ, Hwang JY, Park D, Park CS, Han JY, Chung DY, Park S, Kim EK, Yang SG, Cho SKOncogene , 2026 Apr; 45(14):1234-1246. Epub 2026 Mar 92026 Apr
  • Huber AD, Lin W, Jung YH, Poudel S, Yang G, Wu J, Carrigan AG, Pagala V, Wang W, Fu Y, Yuan ZF, Byrum SD, Yang K, Gee RR, Arnold ED, Loughran AJ, Wang J, Pruett-Miller SM, Peng J, Chen TNat Commun , 2025 Nov 6; 16:9805. Epub 2025 Nov 62025 Nov 6