PE (Phycoerythrin)-conjugated anti-guinea pig antibodies are highly sensitive fluorescent immunoreagents designed for the direct detection of guinea pig immunoglobulins. Phycoerythrin is recognized as one of the brightest fluorophores available, providing exceptional fluorescence intensity with a broad excitation range (496–564 nm) and an emission maximum at approximately 578 nm. These properties make PE conjugates particularly suitable for high-sensitivity fluorescence assays and multicolor analysis with minimal spectral overlap.
Formats and Specificity
PE-conjugated antibodies are available as whole IgG (donkey or rabbit) and F(ab')₂ fragments (rabbit). Whole IgG antibodies recognize both heavy and light chains (H&L) of guinea pig IgG, whereas F(ab')₂ fragments lack the Fc region, reducing non-specific Fc receptor binding and improving performance in live-cell staining. Specificity is verified by immunoelectrophoresis, demonstrating strong reactivity with guinea pig IgG and light chains while showing no detectable cross-reactivity with non-immunoglobulin proteins.
Applications and Advantages
- High-sensitivity detection: Ideal for flow cytometry, immunofluorescence, ELISA, and Western blot applications requiring bright fluorescent signals.
- Optimized performance: Recommended dilutions include Flow Cytometry (1:20–1:250), Immunofluorescence (1:50–1:1,000), ELISA (1:500–1:1,000), and Western Blot (1:300–5,000).
- Reliable multiplex analysis: The exceptional brightness of PE enables accurate detection of low-abundance cell surface markers while supporting multicolor experimental panels with reduced spectral interference.
Storage
These antibodies are typically supplied in PBS or TBS containing BSA and a preservative. Store at 4°C for short-term use or at −20°C with glycerol for long-term storage. To preserve fluorescence intensity and conjugate stability, samples should always be protected from prolonged exposure to light.
