PE-Cy7-conjugated anti-mouse primary antibodies are immunoreagents labeled with PE-Cy7, a tandem fluorochrome composed of phycoerythrin (PE) as the donor and cyanine 7 (Cy7) as the acceptor. PE-Cy7 is excited by the 488 nm or 561 nm laser and emits in the far-red region, approximately 780 nm. These antibodies enable direct, one-step detection of mouse antigens or immunoglobulins. This eliminates secondary reagents, reduces assay time, and provides a valuable far-red channel for multicolor flow cytometry panels with minimal spectral overlap.

Applications

These reagents are used primarily in flow cytometry for immunophenotyping, rare event analysis, and multicolor panels alongside FITC, PE, APC, and Pacific Blue. They are also applicable to fluorescence microscopy with suitable filter sets. PE-Cy7 is moderately bright but susceptible to photobleaching and tandem degradation. It performs well for surface staining, although fixation and permeabilization may affect the tandem complex. It is less suitable for detecting very low-abundance antigens.

Specificity

Specificity is determined by the antibody moiety. Monoclonal antibodies recognize a single epitope, while polyclonal antibodies bind multiple epitopes. Cross-adsorption minimizes cross-species reactivity. Conjugation may slightly alter avidity or brightness. Isotype, unstained, and compensation controls are essential. Store protected from light, avoid freeze-thaw cycles, and validate each lot for the intended application to ensure reproducible, target-specific detection.