Clearing and deparaffinization agents for histology
The next step after dehydration is called "clearing" and consists of replacing the dehydration solution with a substance that will be miscible with the inclusion medium (paraffin). The term "clearing" comes from the fact that clarifying agents often have the same refractive index as proteins. As a result, when the tissue is completely infiltrated with the clarifier, it becomes translucent. This change in appearance is often used as an indication of the effectiveness of the process.
The clarifying and deparaffinization agents are completely miscible in alcohol and paraffin, which is why these solutions are widely used in histology and cytology. Many steps in the preparation and staining of tissue sections involve clarifying agents, such as, for example, prior to paraffin embedding, when paraffin is removed from the tissue section prior to staining and before mounting between blade and coverslip with mounting medium for long-term observation and storage.
Usually the clarification and dewaxing solutions are based on xylene or toluene. However, given the toxicity of these solvents, there are now alternatives based on hydrocarbons which have the advantage of being much less toxic than xylene but also to be odorless.
Frequently Asked Questions (FAQ)
Where can I find clearing and deparaffinization agents products on the Quimigen website?
clearing and deparaffinization agents products are available in this section and can be sorted by product families or sub-categories, such as Xylene, Substitutes. You can browse the catalogue tree or use the search bar with a product name, catalogue reference, brand or supplier.
What types of products are available in the clearing and deparaffinization agents category?
The clearing and deparaffinization agents category brings together various product references, consumables, reagents, kits, instruments or laboratory solutions, depending on the relevant product family. The Xylene, Substitutes sub-categories allow you to find the products that meet your needs more quickly.
How can I quickly find a clearing and deparaffinization agents product?
To find a clearing and deparaffinization agents product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.
Does Quimigen stock several product codes in the clearing and deparaffinization agents category?
Yes, Quimigen distributes several product references in the clearing and deparaffinization agents category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.
Can I request a quote for clearing and deparaffinization agents products?
Yes, you can request a quote for clearing and deparaffinization agents products from the product page or by contacting Quimigen (info@quimigen.pt). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.
How can I find out if a clearing and deparaffinization agents product is available to order?
The availability of a clearing and deparaffinization agents product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact Quimigen with the product code or name of the product you are looking for.
What should I do if I cannot find the clearing and deparaffinization agents product I am looking for?
If the clearing and deparaffinization agents product you are looking for does not appear in the catalogue, please contact Quimigen with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.
Are all clearing and deparaffinization agents products available under the same terms and conditions?
No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.