Alkaline Phosphatase (AP)-conjugated Anti-Guinea Pig primary antibodies are enzyme-labeled secondary detection reagents used for identifying guinea pig immunoglobulins or guinea pig-derived targets in ELISA, Western blot, and related immunoassays. The alkaline phosphatase label enables direct colorimetric detection following substrate addition, providing a sensitive and straightforward readout.
Specifications
Typical preparations are affinity-purified polyclonal antibodies and supplied in buffered glycerol or liquid stabilizing solutions. Datasheets usually report conjugate activity, concentration, storage conditions, and recommended working dilutions for ELISA and Western blot applications.
Applications
These antibodies are widely used in direct ELISA, Western blot, dot blot, and related solid-phase assays due to the robust enzymatic activity of alkaline phosphatase. Upon addition of substrates such as pNPP or BCIP/NBT, a stable colorimetric signal is generated, enabling straightforward protein detection.
They provide economical, stable, and highly visible detection, making them suitable for routine laboratory workflows where reproducibility, sensitivity, and straightforward colorimetric readout are required in immunoassays and blotting applications systems.