ABC detection kits - Sheep - Alkaline phosphatase (AP)

ABC detection kits - Sheep - Alkaline phosphatase (AP)

 

The principle of ABC detection kits for IHC is based on the affinity of avidin (or streptavidin) for biotin. Indeed, abbreviation ABC stands for Avidin / Biotin Complex.
The Anti-Sheep IgG ABC detection kits are designed to detect primary sheep antibodies by immunohistochemistry:
  • Biotinylated secondary antibodies to sheep IgG
  • Avidin / AP complex (Alkaline Phosphatase)
The kits may or may not contain the blocking and dilution buffers and the substrate depending on the formats. In general, bulk kits (larger volumes) do not include the substrate.
The most commonly used alkaline phosphatase substrates are Fast Red and Permanent Red. These 2 substrates form water-insoluble red precipitates when hydrolysed by alkaline phosphatase (AP). It is for this reason that they are commonly called chromogens. The localization of the target proteins is then carried out by microscopic visualization of the precipitates within the tissue.

 

Frequently Asked Questions (FAQ)

How can I quickly find a alkaline phosphatase (ap) product?

To find a alkaline phosphatase (ap) product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.

Does Quimigen stock several product codes in the alkaline phosphatase (ap) category?

Yes, Quimigen distributes several product references in the alkaline phosphatase (ap) category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.

Can I request a quote for alkaline phosphatase (ap) products?

Yes, you can request a quote for alkaline phosphatase (ap) products from the product page or by contacting Quimigen (info@quimigen.pt). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.

How can I find out if a alkaline phosphatase (ap) product is available to order?

The availability of a alkaline phosphatase (ap) product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact Quimigen with the product code or name of the product you are looking for.

What should I do if I cannot find the alkaline phosphatase (ap) product I am looking for?

If the alkaline phosphatase (ap) product you are looking for does not appear in the catalogue, please contact Quimigen with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.

Are all alkaline phosphatase (ap) products available under the same terms and conditions?

No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.

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