ABC detection kits - Anti-Mouse IgG
The principle of ABC detection kits is based on the affinity of avidin (or streptavidin) for biotin. Indeed, abbreviation ABC stands for Avidin / Biotin Complex.
Avidin is a 68 kDa basic glycoprotein found in egg white as well as tissues of birds, reptiles and amphibians. Avidin has a strong affinity for biotin, a soluble vitamin of 34 kDa. Biotin can be coupled to a wide variety of biological molecules such as antibodies, and several biotin molecules can be linked to a protein. The biotinylated protein may then bind to more than one avidin molecule. A disadvantage of avidin is the non-specific binding to negatively charged structures as the nucleus of the cells. The use of streptavidin greatly reduces this non-specific binding.
Streptavidin is isolated from Streptomyces avidinii and is similar in size and affinity to avidin biotin. But unlike avidin, streptavidin is not glycosylated which makes proteins less prone to nonspecific binding in immunohistochemical applications.
ABC anti-mouse detection kits are designed for the detection of mouse IgGs :
- Biotinylated secondary antibodies anti-mouse IgG
- An Avidin / Enzyme Complex (HRP or AP)
The kits may or may not contain the blocking and dilution buffers and the substrate depending on the formats.
Frequently Asked Questions (FAQ)
Where can I find anti-mouse products on the Quimigen website?
anti-mouse products are available in this section and can be sorted by product families or sub-categories, such as Horseradish Peroxidase (HRP), Alkaline phosphatase (AP). You can browse the catalogue tree or use the search bar with a product name, catalogue reference, brand or supplier.
What types of products are available in the anti-mouse category?
The anti-mouse category brings together various product references, consumables, reagents, kits, instruments or laboratory solutions, depending on the relevant product family. The Alkaline phosphatase (AP), Horseradish Peroxidase (HRP) sub-categories allow you to find the products that meet your needs more quickly.
How can I quickly find a anti-mouse product?
To find a anti-mouse product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.
Does Quimigen stock several product codes in the anti-mouse category?
Yes, Quimigen distributes several product references in the anti-mouse category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.
Can I request a quote for anti-mouse products?
Yes, you can request a quote for anti-mouse products from the product page or by contacting Quimigen (info@quimigen.pt). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.
How can I find out if a anti-mouse product is available to order?
The availability of a anti-mouse product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact Quimigen with the product code or name of the product you are looking for.
What should I do if I cannot find the anti-mouse product I am looking for?
If the anti-mouse product you are looking for does not appear in the catalogue, please contact Quimigen with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.
Are all anti-mouse products available under the same terms and conditions?
No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.