Texas Red®-conjugated anti-mouse primary antibodies are directly labeled immunoreagents in which a mouse antigen-specific immunoglobulin is covalently linked to the Texas Red sulfonyl chloride fluorophore. Unlike PE-Texas Red tandem reagents, which are often secondary antibodies, these direct conjugates bind mouse antigens—such as CD4, CD8a, CD45, CD3e, and F4/80—in a single staining step. Texas Red exhibits excitation and emission maxima of approximately 595–596 nm and 613–620 nm, respectively, with a small Stokes shift. It is optimally excited by the 561 nm or 594 nm laser lines and detected with 610/20 nm or 615/30 nm bandpass filters. Direct labeling eliminates secondary antibody incubation, reduces background from endogenous mouse immunoglobulins, and improves reproducibility in mouse-on-mouse applications.

Applications

• Flow cytometry: Immunophenotyping of mouse leukocyte subsets when a 561 nm or 594 nm laser is available; useful for multicolor panels alongside FITC, PE, and APC.
• Immunofluorescence microscopy: Sensitive detection of mouse antigens in cultured cells and tissue sections, particularly for dual-labeling with green fluorophores.
• Immunohistochemistry: Staining of frozen and formalin-fixed, paraffin-embedded mouse tissues, including multiplex IHC.
• Western blotting: Fluorescent detection of mouse proteins on membranes.
• Fluorescence-based plate assays (FLISA): Quantitative immunoassays in multiwell formats.