Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence A DNA sequence encoding human full-length AR
Tag	N-GST and C-His
Predicted MW	72.5 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1% sarkosyl, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping	Dry Ice
Reference Data
RefSeq	NP_001011645
Locus ID	367
UniProt ID	P10275
Cytogenetics	Xq12
RefSeq Size	1765
RefSeq ORF	1164
Synonyms	AIS; AR8; DHTR; HUMARA; HYSP1; KD; NR3C4; SBMA; SMAX1; TFM
Summary	The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (SBMA, also known as Kennedy's disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Jan 2017
Protein Categories	Growth Factors, Intracellular Proteins, Nervous system: Neurodegenerative diseases, Transciption Factors
Protein Families	Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
Protein Pathways	Oocyte meiosis, Pathways in cancer, Prostate cancer

FAQs

Protein FAQs

SDS

SDS for TP761906

Recombinant Protein Resources

Video tutorial: Why protein expression host matters Webinar Video: Overexpression lysate as positive controls for Western blot Product Overview Video: Overexpression lysates as positive controls for Western blot Video Tutorial: Knockout(KO) lysate and antibody specificity Product Brochure: Protein

SKU	Description	Size
LC400012	Lyophilized AR HEK293T cell transient overexpression lysate (as WB positive control)	20 ug
LC423281	Lyophilized AR HEK293T cell transient overexpression lysate (as WB positive control)	20 ug
LY400012	Transient overexpression lysate of androgen receptor (AR), transcript variant 1 (as WB positive control)	100 ug
LY423281	Transient overexpression lysate of androgen receptor (AR), transcript variant 2 (as WB positive control)	100 ug