Quantitative antibody arrays on glass slides are multiplex immunoassays designed to measure multiple proteins simultaneously in biological samples. In veterinary and comparative research, species-specific or cross-reactive arrays for equine, ovine, rabbit, porcine, canine and feline samples can support the quantitative profiling of cytokines and other immune-related proteins. Glass-slide arrays typically use spatially immobilized capture antibodies and fluorescent detection. In quantitative formats, calibration standards and standard curves enable estimation of protein concentrations within the validated assay range. Peer-reviewed research demonstrates the utility of multiplex protein profiling for investigating immune responses in domestic animals, while highlighting the need to assess antibody specificity, cross-reactivity and assay performance for each target and species.
Key Features
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Multiplex protein detection: Simultaneous measurement of multiple targets in a single sample.
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Quantitative analysis: Fluorescence-based detection and calibration standards support protein concentration estimation in validated assay formats.
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Species-oriented profiling: Target panels and antibody reactivity vary by species and assay.
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Glass-slide format: Spatially organized antibody spots enable parallel analysis of multiple protein targets.
Usual Research Applications
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Characterization of cytokine profiles during infection, inflammation and immune activation.
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Investigation of host–pathogen interactions and immune responses to experimental treatments or vaccination.
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Comparative immunology and studies of species-specific immune regulation.
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Exploratory biomarker discovery and protein profiling in compatible samples, including serum, plasma and cell culture supernatants.
