Quantitative cytokine antibody arrays on glass slides are multiplex immunoassays designed to measure several cytokines simultaneously in biological samples. For non-human species, including rabbits, dogs, horses, cats, sheep and chickens, these assays can support research into species-specific immune responses, inflammation and host–pathogen interactions. Peer-reviewed studies demonstrate the utility of multiplex cytokine profiling in veterinary and comparative immunology, although available targets, assay formats and species-specific validation vary.
Glass-slide antibody arrays use spatially immobilized capture antibodies to detect selected cytokines through specific antigen–antibody interactions. Quantitative formats use calibration standards to estimate cytokine concentrations within the assay's validated measurement range, whereas semi-quantitative arrays generally compare relative signal intensities between samples. These formats should not be considered interchangeable.
Key Features
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Multiplex detection: Parallel analysis of multiple cytokines from a single sample.
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Glass-slide format: Spatially arranged capture antibodies enable simultaneous detection of selected targets.
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Quantitative analysis: Concentration estimates based on calibration standards, where supported by the assay design.
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Species-dependent coverage: Target panels, antibody specificity and validation vary by species.
Usual Research Applications
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Investigating cytokine secretion and immune regulation.
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Profiling inflammatory responses to infection, vaccination or experimental stimulation.
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Comparing cytokine patterns across biological samples or experimental conditions.
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Studying veterinary immunology, animal disease models and comparative immunology.
