Lauryl Sulfate Broth is a selective growth medium used for the detection of coliform bacteria in water and wastewater in a laboratory setting. The coliform group includes aerobic and facultative anaerobic, Gram-negative and non-spore-forming bacilli, which ferment lactose and form acid and gas at 35°C in 48 hours. Members of the Enterobacteriacae make up the majority of this group, but organisms such as Aeromonas spp. may also be included. Coliform detection and confirmation procedures are used to test water, food, dairy products and other materials. Enzymatic digestion of casein provides nitrogen, vitamins, minerals and amino acids in Lauryl Tryptose broth. Lactose is the fermentable carbohydrate of coliforms. Phosphates are the buffering agents, and sodium chloride is used to maintain the osmotic balance of the medium. Sodium lauryl sulfate is the selective agent used to inhibit non-coliform organisms.