Human Inflammation Antibody Arrays on Glass Slides are multiplex protein profiling tools designed for the simultaneous detection and semi-quantitative analysis of multiple inflammation-related proteins in human biological samples. Based on antibody microarray technology, they use specific capture antibodies immobilized at defined positions on functionalized glass slides to recognize target inflammatory mediators. Following sample incubation, captured proteins are detected using corresponding detection antibodies and a fluorescence-based detection system, with signal intensities measured by fluorescence microarray scanning. This approach enables the comparative profiling of cytokines, chemokines and other inflammatory mediators involved in immune responses and inflammatory signaling pathways.
Key Features
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Multiplex detection: Simultaneous analysis of multiple inflammation-related proteins, including interleukins (ILs), tumor necrosis factor (TNF), interferons (IFNs) and chemokines.
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Glass-slide format: Spatially defined antibody immobilization enables parallel protein detection while limiting sample consumption.
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Fluorescence-based detection: Signal intensity provides semi-quantitative information about relative protein abundance.
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Comparative profiling: Enables the assessment of differences in inflammatory mediator abundance between experimental conditions.
Usual Research Applications
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Investigating inflammatory and immune response pathways.
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Studying cytokine and chemokine secretion in stimulated cell cultures.
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Comparing inflammatory profiles in serum and other compatible biological samples.
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Exploring inflammation-associated mechanisms in disease models and cellular responses.
