Recombinant SARS-CoV-2 Nucleocapsid Protein with C-terminal His-tag, secreted from the serum-free medium of transfected HEK293 cells. Purchase will also include one vial of normal control (Catalog #. 230-20001), the culture supernatant of HEK293 cell transfected with empty expression vector.
Recombinant SARS-CoV-2 Nucleocapsid Protein with C-terminal His-tag, Transfected HEK293 Cell Culture Supernatant
Recombinant SARS-CoV-2 Nucleocapsid Protein with C-terminal His-tag, Transfected HEK293 Cell Culture Supernatant
Contact Us for Bulk Request
Product Description
Specifications
| Size | 200 µL |
| Species | SARS-CoV-2 |
| Accession Number | QHD43423 |
| Protein Name / Synonyms | SARS-CoV-2 Nucleocapsid Protein |
| Expressed Region | Met1-Ala419 |
| Expression System | HEK293 cells |
| Tag | C-terminal his-tag |
| Purity | Unpurified cell culture supernatant. HEK293 cells grown in serum-free medium were transfected with expression vector harboring target gene. The cell culture was harvested with centrifugation to remove cells. The cell culture supernatant containing mammalian cell protease inhibitor cocktail was aliquoted and stored at -80°C immediately. The gene overexpression in culture supernatant was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the culture supernatant derived from HEK293 cells transfected with the empty expression vector was used as a negative control. |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 50. The actual molecular weight may increase slightly due to the potential post-translational modifications (PTMs). |
| Format | Pink liquid |
| Formulation | Cell culture supernatant of transfected HEK293 cells, serum-free. |
| Preservative | None |
| Research Area | Infectious Disease, COVID-19 |
| Shipping Type | Blue ice |
| Storage | -20°C |
Stability & Storage
Shipping
Including one vial of cell culture supernatant of HEK293 cells transfected with empty expression vector.
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
Related Coronavirus Proteins
Spike Protein, S1 Subunit
230-01101
Spike Protein, S1 Subunit, Host Cell (RBD)
230-01102
Spike Protein, S2 Subunit
230-01103
Nucleocapsid Protein
230-01104
S1 Subunit Protein (RBD) with C-terminal Fc tag
230-20405-200
S1 Subunit Protein (RBD) with C-terminal His-tag
230-20406-200
S1 Subunit Protein with C-terminal His-tag
230-20407-200
S2 Subunit Protein with C-terminal His-tag
230-20408-200
documents
citations
Genomic RNA Elements Drive Phase Separation of the SARS-CoV-2 Nucleocapsid
- Sample Type:
- Purified / Recombinant Protein (recombinant N-protein in vitro phase separation)
- Year:
- 2020
- Journal Name:
- Molecular Cell
- PubMed ID:
- 33290746