Rabbit Anti-PON2 Polyclonal Antibody

Referência HPA029193

Tamanho : 100ul

Marca : Atlas Antibodies

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HPA029193
paraoxonase 2

Anti-PON2 Antibody

Polyclonal Antibody against HUMAN PON2

0.05 mg/ml
info@quimigen.pt

Immunohistochemistry analysis in human cerebral cortex and pancreas tissues using Anti-PON2 antibody. Corresponding PON2 RNA-seq data are presented for the same tissues.

Product details

  • Target protein: paraoxonase 2
  • Target gene: PON2
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000032667 (91%)RAT - ENSRNOG00000009112 (89%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: A106867
  • Concentration: 0.05 mg/ml

Applications

  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:20 - 1:50
    • Retrieval method: HIER pH6
  • Western Blot (WB) recommended conditions
    • Working concentration: 0.04-0.4 µg/ml

Validation

  • Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.  
  • Western Blot (WB)Recombinant expression validation in WB using target protein overexpression. 

Target information

  • Protein name: paraoxonase 2
  • Gene name: PON2
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (2)

  • Shakhparonov MI, Antipova NV, Shender VO, Shnaider PV, Arapidi GP, Pestov NB, Pavlyukov MSActa Naturae , 2018 Jul-Sep; 10(3):92-992018 Jul-Sep

  • This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
    Human Protein Atlas

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