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> Rabbit Anti-FASN Polyclonal Antibody

Rabbit Anti-FASN Polyclonal Antibody

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Referência HPA006461

Tamanho : 100ul

Marca : Atlas Antibodies

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HPA006461
fatty acid synthase

Anti-FASN Antibody

Polyclonal Antibody against HUMAN FASN

0.2 mg/ml
Anti-FASN Antibody
Anti-FASN Antibody
Anti-FASN Antibody
Anti-FASN Antibody
Anti-FASN Antibody
Anti-FASN Antibody
Anti-FASN Antibody
Anti-FASN Antibody
Anti-FASN Antibody
Anti-FASN Antibody
Anti-FASN Antibody
Anti-FASN Antibody
Anti-FASN Antibody
info@quimigen.pt

Immunohistochemistry analysis in human breast and skeletal muscle tissues using Anti-FASN antibody. Corresponding FASN RNA-seq data are presented for the same tissues.

Product details

  • Target protein: fatty acid synthase
  • Target gene: FASN
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000025153 (64%)RAT - ENSRNOG00000045636 (68%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: B90017
  • Concentration: 0.2 mg/ml

Applications

  • Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
    • Working concentration: 0.25-2 µg/ml
    • Fixation/Permeabilization: PFA/Triton X-100
  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:50 - 1:200
    • Retrieval method: HIER pH6
  • Western Blot (WB) recommended conditions
    • Working concentration: 0.04-0.4 µg/ml

Validation

  • Immunofluorescence in Cell Lines (ICC-IF) 
  • Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.  
  • Western Blot (WB)Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein. Validated in Western blot using relevant lysates (see Western blot application images above) 

Target information

  • Protein name: fatty acid synthase
  • Gene name: FASN
  • Alternative gene names: FAS, SDR27X1, 
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (4)

  • Vergani E, Beretta GL, Aloisi M, Costantino M, Corno C, Frigerio S, Tinelli S, Dugo M, Accattatis FM, Granata A, Arnaboldi L, Rodolfo M, Perego P, Gatti LFront Cell Dev Biol , 2022 Jul 13; 10:927118. Epub 2022 Jul 132022 Jul 13
  • Månberg A, Bradley F, Qundos U, Guthrie BL, Birse K, Noël-Romas L, Lindskog C, Bosire R, Kiarie J, Farquhar C, Burgener AD, Nilsson P, Broliden KMol Cell Proteomics , 2019 Mar; 18(3):461-4762019 Mar

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