Mouse matrix metallopeptidase 7 (matrilysin, uterine) (MMP7) ELISA kit
Referência CSB-E07409m-96T
Tamanho : 96T
Marca : Cusabio
Contactar o distribuidor local :
Telefone : +1 850 650 7790
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
matrix metallopeptidase 7 (matrilysin, uterine)
Associated names
Mmp7Matrilysin ELISA Kit; EC 3.4.24.23 ELISA Kit; Matrin ELISA Kit; Matrix metalloproteinase-7 ELISA Kit; MMP-7 ELISA Kit; Pump-1 protease ELISA Kit; Uterine metalloproteinase ELISA Kit
Abbreviation
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.81 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MMP7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 90-95 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 98-104 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 95-102 | |||||
Recovery
| The recovery of mouse MMP7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 92-96 | ||||
| EDTA plasma (n=4) | 96 | 92-99 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Mouse MMP7 ELISA Kit was designed for the quantitative measurement of Mouse MMP7 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL .
Citations
- L Huang,Microchimica Acta,2020
Customer Reviews and Q&A
There are currently no reviews for this product.
■ Q&A
Q:
Does the kit contain human and/or animal by-products? What is the standard made of?
A:
Thanks for your inquiry. Standard is NS0-derived recombinant mouse FBLN1 protein.
The kit does not contain any human and/or animal by-products.
The kit does not contain any human and/or animal by-products.
Target Background
Function
(From Uniprot)
(From Uniprot)
Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.; May play a role in tissue reorganization.
Subcellular Location
Secreted, extracellular space, extracellular matrix.
Protein Families
Peptidase M10A family
Database Links
STRING:
UniGene: