General Information
| Short Description | Mouse monoclonal anti-Actin, cytoplasmic 1 for use in IF, WB and IHC in Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit and Insect samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IF/WB/IHC |
| Host | Mouse |
| Reactivity | Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Insect |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 5B7 |
| Isotype | IgG1 |
| Conjugation | HRP |
| Concentration | 1 mg/mL |
| Purification | The antibody was purified using affinity-chromatography using specific immunogen. |
| Dilution Range | Optimal working dilutions should be determined experimentally by the investigator Suggested starting dilutions are as follows: WB 1:2000-5000 |
| Formulation | Liquid in PBS pH7.4, containing 0.02% Sodium Azide and 50% Glycerol. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | |
| Gene ID | |
| Uniprot ID | |
| Specificity | Beta-actin Monoclonal Antibody (5B7) specially designed for your Western blot as internal control. |
Additional Info
| Function | Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA. Plays a role in the assembly of the gamma-tubulin ring complex (gTuRC), which regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments. Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules. |
| Protein Name | Actin - Cytoplasmic 1Beta-Actin Cleaved Into - Actin - Cytoplasmic 1 - N-Terminally Processed |
| Database Links | |
| Cellular Localisation | CytoplasmCytoskeletonNucleusLocalized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas |
| Alternative Antibody Names | Anti-Actin - Cytoplasmic 1 antibodyAnti-Beta-Actin Cleaved Into - Actin - Cytoplasmic 1 - N-Terminally Processed antibodyAnti-ACTB antibody |
Information sourced from Uniprot.org