Vps34-PIK-III [1383716-40-2]
Referência HY-12794-1ml
Tamanho : 10mM/1mL
Marca : MedChemExpress
Vps34-PIK-III is an orally active and selective VPS34 inhibitor (IC50=18 nM). Vps34-PIK-III effectively inhibits autophagy and can be used as a molecular tool. vps34-PIK-III is also a PI3K inhibitor that inhibits the expression of genes in liver cancer stem cells (CSCs).
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Vps34-PIK-III Chemical Structure
CAS No. : 1383716-40-2
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Based on 5 publication(s) in Google Scholar
Description |
Vps34-PIK-III is an orally active and selective VPS34 inhibitor (IC50=18 nM). Vps34-PIK-III effectively inhibits autophagy and can be used as a molecular tool. vps34-PIK-III is also a PI3K inhibitor that inhibits the expression of genes in liver cancer stem cells (CSCs)[1][2][3]. |
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IC50 & Target[2] |
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In Vitro |
Vps34-PIK-III (1, 5, 10 μM; 24 h) inhibits autophagy and LC3 lipidation in DLD1 cells[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability Assay[1]
RT-PCR[1]
Western Blot Analysis[1]
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In Vivo |
Vps34-PIK-III (10 mg/kg; p.o.; single) rapidly absorbed and shows moderate mean systemic clearance (30 mL/min/kg, approximately 33% of hepatic blood flow), with good oral bioavailability (F% = 47)[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Masse moléculaire |
319.36 |
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Formule |
C17H17N7 |
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CAS No. |
1383716-40-2 |
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Appearance |
Solid |
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Color |
White to off-white |
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SMILES |
NC1=NC=C(C2=CC=NC(NC3=CC=NC=C3)=N2)C(CC4CC4)=N1 |
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Livraison | Room temperature in continental US; may vary elsewhere. |
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Stockage |
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Solvant et solubilité |
In Vitro:
DMSO : ≥ 31 mg/mL (97.07 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) *"≥" means soluble, but saturation unknown. Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Pureté et documentation |
Purity: 99.02% |
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Références |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO | 1 mM | 3.1313 mL | 15.6563 mL | 31.3126 mL | 78.2816 mL |
5 mM | 0.6263 mL | 3.1313 mL | 6.2625 mL | 15.6563 mL | |
10 mM | 0.3131 mL | 1.5656 mL | 3.1313 mL | 7.8282 mL | |
15 mM | 0.2088 mL | 1.0438 mL | 2.0875 mL | 5.2188 mL | |
20 mM | 0.1566 mL | 0.7828 mL | 1.5656 mL | 3.9141 mL | |
25 mM | 0.1253 mL | 0.6263 mL | 1.2525 mL | 3.1313 mL | |
30 mM | 0.1044 mL | 0.5219 mL | 1.0438 mL | 2.6094 mL | |
40 mM | 0.0783 mL | 0.3914 mL | 0.7828 mL | 1.9570 mL | |
50 mM | 0.0626 mL | 0.3131 mL | 0.6263 mL | 1.5656 mL | |
60 mM | 0.0522 mL | 0.2609 mL | 0.5219 mL | 1.3047 mL | |
80 mM | 0.0391 mL | 0.1957 mL | 0.3914 mL | 0.9785 mL |