This kit contains reagents for performing tyramide signal amplification (TSA) using goat anti-mouse HRP secondary antibody and fluorescent CF® Dyes or biotin to increase immunofluorescence signal.
Features
- Increase sensitivity by up to 100-fold
- Use lower primary antibody concentrations
- Boost signal in challenging samples, like human FFPE tissues
- Choose from 6 bright & photostable CF® dyes or biotin
Kit Components:
- CF® dye or biotin-tyramide stock solution
- HRP Goat anti-mouse IgG (highly cross-adsorbed)
- BSA for blocking
- Tyramide Amplification Buffer Plus*
- 30% Hydrogen Peroxide*
Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to conventional avidin-biotin detection. Multiple rounds of tyramide signal amplification can be performed for multiplex detection; to learn more, see our Tech Tip: Multicolor Fluorescence Imaging using Tyramide Amplification Kits. Learn more about tyramide signal amplification.
The HRP goat anti-mouse antibody provided in the kits has been adsorbed against bovine, horse, human, rabbit, and swine serum to minimize cross-reactivity; the antibody is not cross-adsorbed against rat. The kits include your choice of 6 CF® dye tyramides or biotin tyramide. CF® dyes are Biotium's line of next-generation fluorescent dyes, with advantages in brightness and photostability compared to other dyes. Learn more about CF® dyes.
Also see our Tyramide Amplification Kits with HRP Goat Anti-Rabbit and Tyramide Amplification Kits with HRP Streptavidin.
Each kit contains reagents for staining 50-150 slides; the actual number of samples that can be stained will depend on staining volumes used. Tyramide Amplification Buffer, CF® Dye Tyramides, and other tyramide compounds are also available separately.
Also learn about TyraMax™ Amplification Dyes and Kits, Biotium's next generation tyramide dyes that offer brighter signal compared to the original CF® Dye Tyramides, and have advantages in brightness, photostability, and working solution stability compared to other TSA dyes. We also offer Ready-to-Use Tyramide Amplification Buffer and Tyramide Amplification Buffer Plus (an improved formulation for enhanced TSA sensitivity). Learn more about Tyramide Signal Amplification.
Tyramide Signal Amplification Kits
| Tyramide Label | Ex/Em | Secondary conjugate | Catalog no. |
| CF®488A | 490/515 nm | Goat anti-mouse HRP | 33000 |
| Goat anti-rabbit HRP | 33001 |
| Streptavidin HRP | 33002 |
| CF®543 | 541/560 nm | Goat anti-mouse HRP | 33003 |
| Goat anti-rabbit HRP | 33004 |
| Streptavidin HRP | 33005 |
| CF®568 | 562/583 nm | Goat anti-mouse HRP | 33006 |
| Goat anti-rabbit HRP | 33007 |
| Streptavidin HRP | 33008 |
| CF®594 | 593/614 nm | Goat anti-mouse HRP | 33009 |
| Goat anti-rabbit HRP | 33010 |
| Streptavidin HRP | 33011 |
| CF®640R | 642/662 nm | Goat anti-mouse HRP | 33012 |
| Goat anti-rabbit HRP | 33013 |
| Streptavidin HRP | 33014 |
| CF®680R | 680/701 nm | Goat anti-mouse HRP | 33015 |
| Goat anti-rabbit HRP | 33016 |
| Streptavidin HRP | 33017 |
| Biotin-XX | N/A | Goat anti-mouse HRP | 33018 |
| Goat anti-rabbit HRP | 33019 |
| Streptavidin HRP | 33020 |
*Note: Older versions of this kit contain Ready-to-Use Tyramide Amplification Buffer (component 22027-25mL) without a separate vial of hydrogen peroxide. Download the product information sheet for detailed protocols. The SDS for the older version of the kit can be downloaded here:
SDS-Tyramide-Amplification-Kit with 22027.
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