Tet1 Antibody - C-terminal region
Referência OAAB19195
Tamanho : 400ul
Marca : Aviva Systems Biology
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Tet1 Antibody - C-terminal region (OAAB19195)
| Datasheets/Manuals | Printable datasheet for Tet1 Antibody - C-terminal region (OAAB19195) |
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| Predicted Species Reactivity | Human|Mouse |
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| Product Format | Liquid. PBS with 0.09% (W/V) sodium azide. |
| Clonality | Polyclonal |
| Isotype | Rabbit Ig |
| Host | Rabbit |
| Application | WB |
| Additional Information | Function: Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5- hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Also mediates subsequent conversion of 5hmC into 5- formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, plays a more general role in chromatin regulation. Preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and Polycomb-repressed genes. Involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. Also involved in transcription repression of a subset of genes through recruitment of transcriptional repressors to promoters. Involved in the balance between pluripotency and lineage commitment of cells it plays a role in embryonic stem cells maintenance and inner cell mass cell specification. Cellular Location: Nucleus. Tissue Location Present in embryonic stem cells (ES cells). |
| Reconstitution and Storage | Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Immunogen | This mouse Tet1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1845-1879 amino acids from the C-terminal region of mouse Tet1. |
| Purification | This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Concentration | 0.5 mg/ml |
| Intended Use | For research use only and not for use in diagnostic or therapeutic procedures. |
| Application Info | WB: 1:2000 |
| Gene Symbol | TET1 |
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| Alias Symbols | 2510010B09Rik, AA517754, BB001228, CXXC finger 6, CXXC-type zinc finger protein 6, Cxxc6, D10Ertd17, D10Ertd17e, LCX, methylcytosine dioxygenase TET1, mKIAA1676, ten-eleven translocation 1 gene protein homolog, tet oncogene 1. |
| NCBI Gene Id | 52463 |
| Protein Name | Methylcytosine dioxygenase TET1 |
| Description of Target | Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, plays a more general role in chromatin regulation. Preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and Polycomb-repressed genes. Involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. Also involved in transcription repression of a subset of genes through recruitment of transcriptional repressors to promoters. Involved in the balance between pluripotency and lineage commitment of cells it plays a role in embryonic stem cells maintenance and inner cell mass cell specification. Plays an essential role in the tumorigenicity of glioblastoma cells. TET1-mediated production of 5hmC acts as a recruitment signal for the CHTOP-methylosome complex to selective sites on the chromosome, where it methylates H4R3 and activates the transcription of genes involved in glioblastomagenesis (PubMed:25284789). |
| Uniprot ID | Q3URK3 |
| Molecular Weight | 222945 |
