Wild-type (WT) and TDG knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TDG antibody (GTX110473) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

TDG antibody detects TDG protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TDG protein stained by TDG antibody (GTX110473) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDG antibody (GTX110473) diluted at 1:500.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDG antibody (GTX110473) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.